RTVP-1, a novel human gene with sequence similarity to genes of diverse species, is expressed in tumor cell lines of glial but not neuronal origin
RTVP-1, a novel human gene with sequence similarity to genes of diverse species, is expressed in tumor cell lines of glial but not neuronal origin
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DOI：
10.1016/s0378-1119(96)00431-3
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发表时间：
1996-11-21
期刊：
影响因子：
3.5
通讯作者：
Israel, MA
中科院分区：
文献类型：
--
作者：
Rich, T;Chen, PC;Israel, MA
从人脑胶质母细胞瘤细胞系U-251 MG的cDNA文库中分离到一个新的基因RTVP-1，该基因与哺乳动物睾丸特异蛋白、植物病程相关蛋白家族和水泡性毒液变应原抗原-5具有显著的序列同源性。序列同源性最高的是人类睾丸特异蛋白TPX1(119个氨基酸中的38.7%)。Northern杂交分析表明，RTVP-1RNA在胎儿组织中仅在肾脏中检测到，但在包括脑在内的成人组织中普遍表达。由RTVP-1编码的多个mRNAs在神经胶质细胞来源的神经系统肿瘤细胞系中高表达，而在非神经系统肿瘤细胞系中低表达或不表达。该序列的GenBank DNA数据库登录号为X91911。
A novel gene, RTVP-1, which shows significant sequence identity to the mammalian testis-specific proteins, a family of plant pathogenesis-related proteins and the vespid venom allergen, antigen-5, has been isolated from a cDNA library of the human glioblastoma brain tumor cell line, U-251 MG. The highest degree of sequence identity was with the human testis-specific protein, TPX1 (38.7% over 119 amino acids). Northern hybridization analysis revealed that in fetal tissue RTVP-1 RNA was detected only in the kidney, but its expression was ubiquitous in adult tissues including brain. Multiple mRNAs encoded by RTVP-1 were highly expressed in a panel of cell lines from nervous system tumors arising from glia, although expression was low or absent in nonglial-derived nervous system tumour cell lines. The GenBank DNA database accession number for this sequence is X91911.