MiR-320a effectively suppresses lung adenocarcinoma cell proliferation and metastasis by regulating STAT3 signals
MiR-320a effectively suppresses lung adenocarcinoma cell proliferation and metastasis by regulating STAT3 signals
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MiR-320a通过调节STAT3信号有效抑制肺腺癌细胞增殖和转移
DOI：
10.1080/15384047.2017.1281497
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发表时间：
2017-01-01
影响因子：
3.6
通讯作者：
Xie, Shu-Yang
中科院分区：
文献类型：
--
作者：
Lv, Qing;Hu, Jin-Xia;Xie, Shu-Yang
摘要MicroRNA在多种肿瘤的发生发展中起着重要作用。miR-320 a对某些肿瘤细胞的增殖有抑制作用，但miR-320 a在肺癌中的生物学作用有待进一步研究。在此，我们研究了miR-320 a在抑制肺腺癌细胞增殖中的作用。MiR-320 a处理被发现有效地抑制LTEP-a-2和A549细胞增殖，并且与对照处理相比，辐射处理诱导更多的凋亡细胞。我们的研究结果还表明，miR-320 a作为一种新的miRNA，直接调节信号转导和转录激活因子3（STAT 3）及其信号，如Bcl-2，Bax和Caspase 3。siRNA抑制的STAT 3水平进一步证明了其在调节STAT 3信号中的作用。此外，与对照组相比，miR-320 a治疗有效地抑制了小鼠异种移植物中的癌细胞生长，并在体外和体内显著抑制了细胞迁移。我们的研究结果共同表明，miR-320 a，通过直接调节STAT 3信号，不仅抑制细胞增殖和转移，但也增强辐射诱导的腺癌细胞凋亡。
ABSTRACT MicroRNAs play important roles in tumorigenesis of various types of cancers. MiR-320a can inhibits cell proliferation of some cancers, but the biologic roles of miR-320a in lung cancer need to be further studied. Here, we investigated the roles of miR-320a in suppressing the proliferation of lung adenocarcinoma cells. MiR-320a treatment was found to effectively suppress LTEP-a-2 and A549 cell proliferation, and induce more apoptotic cells with irradiation treatment compared with control treatment. Our results also showed that miR-320a, as a novel miRNA, directly regulated signal transducer and activator of transcription 3 (STAT3) and its signals, such as Bcl−2, Bax, and Caspase 3. The siRNA-inhibited STAT3 levels further proved its roles in regulating STAT3 signals. Moreover, miR-320a treatment effectively suppressed cancer cell growth in mice xenografts compared with controls, and significantly inhibited cell migration in vitro and in vivo. Our findings collectively demonstrated that miR-320a, by directly regulating STAT3 signals, not only suppressed cell proliferation and metastasis, but also enhanced irradiation-induced apoptosis of adenocarcinomia cells.