Detecting DNA and RNA and Differentiating Single-Nucleotide Variations via Field-Effect Transistors
Detecting DNA and RNA and Differentiating Single-Nucleotide Variations via Field-Effect Transistors
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DOI：
10.1021/acs.nanolett.0c01971
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发表时间：
2020-08-12
期刊：
影响因子：
10.8
通讯作者：
Weiss, Paul S.
中科院分区：
文献类型：
--
作者：
Cheung, Kevin M.;Abendroth, John M.;Weiss, Paul S.
我们使用无标记的电子场效应晶体管(FET)来检测短寡核苷酸，并区分不同单个碱基的序列。我们的传感平台采用单链DNA(SsDNA)化学功能化的超薄膜氧化铟FET。FET中的单链DNA功能化半导体通道检测完全互补的DNA序列，并将这些序列与具有不同类型和位置的单碱基对错配的序列区分开来。由于DNA双链稳定性的差异，与表面结合的单链DNA和双链DNA(DsDNA)相关的电荷变化改变了FET通道的电导，从而使检测成为可能。我们说明了单链DNA-FETs检测互补RNA序列的能力，并与单核苷酸变异的RNA序列区分开来。快速、灵敏地检测和区分寡核苷酸的电子生物传感器的开发和实施为疾病诊断和精确医学领域提供了新的机遇。
We detect short oligonucleotides and distinguish between sequences that differ by a single base, using label-free, electronic field-effect transistors (FETs). Our sensing platform utilizes ultrathin-film indium oxide FETs chemically functionalized with single-stranded DNA (ssDNA). The ssDNA-functionalized semiconducting channels in FETs detect fully complementary DNA sequences and differentiate these sequences from those having different types and locations of single base-pair mismatches. Changes in charge associated with surface-bound ssDNA vs double-stranded DNA (dsDNA) alter FET channel conductance to enable detection due to differences in DNA duplex stability. We illustrate the capability of ssDNA-FETs to detect complementary RNA sequences and to distinguish from RNA sequences with single nucleotide variations. The development and implementation of electronic biosensors that rapidly and sensitively detect and differentiate oligonucleotides present new opportunities in the fields of disease diagnostics and precision medicine.