Innate immune responses induced by the saponin adjuvant Matrix-M in specific pathogen free pigs.
Innate immune responses induced by the saponin adjuvant Matrix-M in specific pathogen free pigs.
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DOI：
10.1186/s13567-017-0437-2
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发表时间：
2017-05-22
影响因子：
4.4
通讯作者：
Fossum C
中科院分区：
文献类型：
--
作者：
Ahlberg V;Hjertner B;Wallgren P;Hellman S;Lövgren Bengtsson K;Fossum C
基于皂苷的佐剂已广泛用于增强许多物种的体液和细胞免疫反应，但其作用方式尚不完全清楚。使用淋巴细胞、单核细胞或单核细胞衍生的树突状细胞 (MoDC) 在体外和体内对猪对 Matrix-M 的转录反应进行表征。 Matrix-M 的效果也在暴露于传统饲养猪的无特定病原体 (SPF) 猪中进行了评估。 Matrix-M 暴露后，单核细胞和淋巴细胞中促炎细胞因子基因 IL1B 和 CXCL8 上调。 Matrix-M 还诱导淋巴细胞中的 IL12B、IL17A 和 IFNG 以及 MoDC 中的 IFN-α 基因表达。注射后 18 小时，血液中的几个基因被 Matrix-M 上调，其中 IFN-α 和 TLR2 基因可以得到统计证实。所有 SPF 猪与常规猪混养后 1-3 天内都会出现呼吸道疾病。四分之二的 SPF 猪在接触暴露前注射了盐水，表现出全身症状，而注射 Matrix-M 的四头猪则没有记录到这一点。粒细胞计数、血清淀粉样蛋白 A 水平以及 IL18 和 TLR2 的转录与猪的疾病进展相符。这些结果支持进一步评估 Matrix-M 作为猪管理关键时刻天然免疫反应的可能增强剂。本文的在线版本 (doi:10.1186/s13567-017-0437-2) 包含补充材料，可供授权用户使用。
Saponin-based adjuvants have been widely used to enhance humoral and cellular immune responses in many species, but their mode of action is not fully understood. A characterization of the porcine transcriptional response to Matrix-M was performed in vitro using lymphocytes, monocytes or monocyte-derived dendritic cells (MoDCs) and in vivo. The effect of Matrix-M was also evaluated in specific pathogen free (SPF) pigs exposed to conventionally reared pigs. The pro-inflammatory cytokine genes IL1B and CXCL8 were up-regulated in monocytes and lymphocytes after Matrix-M exposure. Matrix-M also induced IL12B, IL17A and IFNG in lymphocytes and IFN-α gene expression in MoDCs. Several genes were indicated as up-regulated by Matrix-M in blood 18 h after injection, of which the genes for IFN-α and TLR2 could be statistically confirmed. Respiratory disease developed in all SPF pigs mixed with conventional pigs within 1–3 days. Two out of four SPF pigs injected with saline prior to contact exposure displayed systemic symptoms that was not recorded for the four pigs administered Matrix-M. Granulocyte counts, serum amyloid A levels and transcription of IL18 and TLR2 coincided with disease progression in the pigs. These results support further evaluation of Matrix-M as a possible enhancer of innate immune responses during critical moments in pig management. The online version of this article (doi:10.1186/s13567-017-0437-2) contains supplementary material, which is available to authorized users.