In Salvia miltiorrhiza, phenolic acids possess protective properties against amyloid β-induced cytotoxicity, and tanshinones act as acetylcholinesterase inhibitors
In Salvia miltiorrhiza, phenolic acids possess protective properties against amyloid β-induced cytotoxicity, and tanshinones act as acetylcholinesterase inhibitors
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DOI：
10.1016/j.etap.2011.02.006
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发表时间：
2011-05-01
影响因子：
4.3
通讯作者：
Chen, Lvyi
中科院分区：
文献类型：
--
作者：
Zhou, Yongqiang;Li, Weize;Chen, Lvyi
丹参（Radix Salvia miltiorrhiza，RSM）是一种传统的中草药，据称对老年性痴呆（Alzheimer's disease，AD）具有治疗作用。然而，迄今为止尚未深入研究RSM中主要组分对淀粉样β肽（A β）诱导的细胞毒性和乙酰胆碱酯酶活性的影响。本文以PC-12细胞为模型，研究丹参水/醇提取物、丹参总多酚、丹参总酮和3种酚类化合物对A β（25-35）介导的细胞毒性的影响。丹参水提物和丹参总多酚对A β（25-35）诱导的PC-12细胞毒性有明显的拮抗作用，丹参素和丹参素B B对A β（25-35）诱导的PC-12细胞Ca ~（2+）摄入、乳酸脱氢酶释放、细胞活力下降和细胞凋亡有明显的保护作用。此外，以大鼠脑组织匀浆为酶源，研究了丹参提取物及其相关成分对乙酰胆碱酯酶的抑制活性。氢溴酸加兰他敏是一种公认的乙酰胆碱酯酶抑制剂，用作阳性对照剂。初步研究表明丹参醇提物、丹参总酮、丹参酮I和二氢丹参酮I对乙酰胆碱酯酶有明显的抑制作用。这些研究结果表明丹参中的丹参酮和多酚是该草药治疗AD的有益作用的活性成分。(C)2011 Elsevier B. V.保留所有权利。
Radix Salvia miltiorrhiza (RSM), a traditional Chinese medicinal herb, has been alleged to possess therapeutic effects against senile dementia, also known as Alzheimer's disease (AD). However, the effects of the major components in RSM on cytotoxicity induced by amyloid-beta peptide (A beta) and on acetylcholinesterase activity have not been studied in depth to date. In this report, the effects of RSM aqueous/ethanol extracts, total polyphenols, total tanshinones and 3 phenolic compounds against toxicity mediated by A beta(25-35) were tested with PC-12 cells. The results showed that A beta(25-35)-induced cytotoxicity was revised by RSM aqueous/ethanol extracts and total polyphenols and that danshensu and salvianolic acid B could protect PC-12 cells by blocking A beta(25-35)-induced Ca2+-intake, lactate dehydrogenase release, cell viability decrease and apoptosis. In addition, the activities of RSM extracts and relevant constituents in their inhibition of acetylcholinesterase were investigated using rat brain homogenates as an enzyme resource. Galanthamine hydrobromide, an accepted acetylcholinesterase inhibitor, was employed as a positive control agent. Our preliminary studies demonstrated that RSM ethanol extract, total tanshinones, tanshinone I and dihydrotanshinone I had remarkable inhibition effects on acetylcholinesterase in vitro. These findings suggest that both tanshinones and polyphenols in RSM are the active constituents responsible for the beneficial effects of this herb in AD treatment. (C) 2011 Elsevier B.V. All rights reserved.