CIS-ACTING CONTROL ELEMENTS FOR KRUPPEL EXPRESSION IN THE DROSOPHILA EMBRYO
CIS-ACTING CONTROL ELEMENTS FOR KRUPPEL EXPRESSION IN THE DROSOPHILA EMBRYO
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DOI：
10.1002/j.1460-2075.1990.tb07440.x
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发表时间：
1990-08-01
期刊：
影响因子：
11.4
通讯作者：
JACKLE, H
中科院分区：
文献类型：
--
作者：
HOCH, M;SCHRODER, C;JACKLE, H
Krueppel （Kr）是果蝇的一个间隙基因，在胚胎发生的不同阶段表现出复杂的空间表达模式。为了鉴定正常Kr基因表达所需的顺式作用序列，我们分析了转基因胚胎中融合基因构建的表达模式。在这些结构中，细菌lacZ的表达受到位于基础启动子前面的Kr序列的控制。我们确定了顺式作用的Kr控制单元驱动。β。-半乳糖苷酶在早期和晚期胚胎中10个已知位点的表达。不止一种顺式调控元件驱动胚层前区、神经系统、中线前体细胞和羊膜中的表达。此外，两个顺式作用元件在胚皮胚胎的中心区域以条带亚模式指导Kr的第一次合子表达。这两种元件都对母体组织者基因活性的改变做出反应，这些基因是在野生型胚胎中建立胸腹前段时表达Kr所必需的。
Krueppel (Kr), a gap gene of Drosophila, shows complex spatial patterns of expression during the different stages of embryogenesis. In order to identify cis-acting sequences required for normal Kr gene expression, we analyzed the expression patterns of fusion gene constructs in transgenic embryos. In these constructs, bacterial lacZ expression was placed under the control of Kr sequences in front of a basal promoter. We identified cis-acting Kr control units which drive .beta.-galactosidase expression in 10 known locations of Kr expression in early and late embryos. More than one cis-regulatory element drives the expression in the anterior domain at the blastoderm stage, in the nervous system, the midline precursor cells and in the amnio-serosa. In addition, two cis-acting elements direct the first zygotic expression of Kr in a stripped subpattern within the central region of the blastoderm embryo. Both elements respond to alterations in the activities of maternal organizer genes known to be required for Kr expression in establishing the thoracic and anterior abdominal segments in the wild-type embryo.