Propionibacterium Acnes Acts as an Adjuvant in in Vitro Immunization of Human Peripheral Blood Mononuclear Cells
Propionibacterium Acnes Acts as an Adjuvant in in Vitro Immunization of Human Peripheral Blood Mononuclear Cells
复制标题
DOI：
10.1271/bbb.70159
复制
发表时间：
2007-08
期刊：
影响因子：
--
通讯作者：
Y. Jung;S. Matsumoto;M. Yamashita;Kosuke Tomimatsu;K. Teruya;Y. Katakura;S. Shirahata
中科院分区：
文献类型：
--
作者：
Y. Jung;S. Matsumoto;M. Yamashita;Kosuke Tomimatsu;K. Teruya;Y. Katakura;S. Shirahata
我们已经建立了一种体外免疫方案，即人外周血单个核细胞（PBMCs）最初用l-亮氨酸甲酯（LLME）处理，随后在白细胞介素(IL)-2、IL-4和佐剂的存在下用抗原致敏。这种方法产生了抗原特异性抗体。经LLME治疗后，pbmc与外源抗原发生反应。我们正在使用该系统来研究添加剂的免疫调节活性。在本研究中，我们旨在评估痤疮丙酸杆菌（P. acnes）的免疫调节活性，这种已知的免疫调节作用在小鼠模型中表现出多种免疫调节作用。痤疮假单胞杆菌被发现增加PBMC抗原特异性抗体的产生，可能是通过增加炎症细胞因子的产生和/或增加T-B细胞的相互作用。因此，痤疮假单胞杆菌似乎在体外免疫的抗体应答中起佐剂作用。
We have established an in vitro immunization protocol whereby human peripheral blood mononuclear cells (PBMCs) are initially treated with L-leucyl-L-leucine methyl ester (LLME) and subsequently sensitized with antigen in the presence of interleukin (IL)-2, IL-4, and adjuvant. This protocol resulted in the production of antigen-specific antibodies. PBMCs are potentiated to react with exogenous antigens upon treatment with LLME. We are using this system to investigate the immunomodulatory activity of additives. In the present study, we aimed to evaluate the immunomodulatory activity of Propionibacterium acnes (P. acnes), which is known to exhibit various immunomodulatory effects in murine models, using this in vitro immunization protocol. P. acnes was found to augment the production of antigen-specific antibodies by PBMC, possibly through increased production of inflammatory cytokines and/or increased T-B cell interaction. P. acnes hence appears to act as an adjuvant in the antibody response in in vitro immunization.