Oncostatin M: a growth regulator produced by differentiated histiocytic lymphoma cells.
Oncostatin M: a growth regulator produced by differentiated histiocytic lymphoma cells.
复制标题
制瘤素 M：由分化的组织细胞淋巴瘤细胞产生的生长调节剂。
DOI：
10.1073/pnas.83.24.9739
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发表时间：
1986
影响因子：
11.1
通讯作者：
G. Todaro
中科院分区：
文献类型：
--
作者：
J. Zarling;M. Shoyab;H. Marquardt;M. B. Hanson;M. Lioubin;G. Todaro
一种名为制瘤素M的多肽可抑制A375黑色素瘤和其他人类肿瘤细胞的复制，但不抑制正常人类成纤维细胞的复制，该多肽已从U-937组织细胞淋巴瘤细胞的无血清上清液中分离出来，这些细胞在处理后已被诱导分化为巨噬细胞样细胞佛波醇酯佛波醇12-肉豆蔻酸酯13-乙酸酯。在未处理的U-937细胞的上清液中未检测到这种生长抑制活性，表明该蛋白质在佛波酯诱导的分化细胞中被诱导或表达增加。抑瘤素M在pH 2和11之间以及在56 ℃下加热1小时后是稳定的，但在90 ℃下不稳定。制瘤素M的纯化已经通过凝胶色谱和反相HPLC实现，在三氟乙酸水溶液的存在下，依次使用乙腈和正丙醇。制瘤素M的表观分子量约为18，000（用凝胶色谱法测定）和28，000（用聚丙烯酰胺凝胶电泳法测定）。已测定纯化多肽的氨基端氨基酸序列。抑瘤素M和其他蛋白质之间没有发现实质性的序列同源性，包括单核细胞产生的其他肿瘤细胞抑制蛋白。因此，制瘤素M似乎代表一种独特的细胞生长调节剂。
A polypeptide termed oncostatin M, which inhibits the replication of A375 melanoma and other human tumor cells, but not normal human fibroblasts, has been isolated from serum-free supernatants of U-937 histiocytic lymphoma cells that have been induced to differentiate into macrophage-like cells following treatment with the phorbol ester phorbol 12-myristate 13-acetate. No such growth inhibitory activity is detected in the supernatant of untreated U-937 cells, indicating that the protein is induced or increased in expression in the phorbol ester-induced differentiated cells. Oncostatin M is stable between pH 2 and 11 and after heating for 1 hr at 56 degrees C but is not stable at 90 degrees C. Purification of oncostatin M has been achieved by gel chromatography and reversed-phase HPLC, using sequentially acetonitrile and n-propanol in the presence of aqueous trifluoroacetic acid. The apparent molecular weight of oncostatin M is approximately 18,000, as determined by gel chromatography, and 28,000, as determined by polyacrylamide gel electrophoresis. The amino-terminal amino acid sequence of the purified polypeptide has been determined. No substantial sequence homology between oncostatin M and other proteins was found, including other tumor cell inhibitory proteins produced by mononuclear cells. Oncostatin M, therefore, appears to represent a distinct cell growth regulator.