Mycobacterium tuberculosis and gyrA variation in Zambia.
Mycobacterium tuberculosis and gyrA variation in Zambia.
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DOI：
10.2149/tmh.33.91
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发表时间：
2005-01-01
影响因子：
4.5
通讯作者：
Numazaki, Y.
中科院分区：
文献类型：
--
作者：
Mitarai, S.;Kafwabulula, L. M.;Numazaki, Y.
对赞比亚卢萨卡大学教学医院胸科诊所 16 名肺结核患者分离的结核分枝杆菌菌株的氟喹诺酮敏感性和分子流行病学进行了调查[日期未给出]。测定分离株对异烟肼、利福平、乙胺丁醇和链霉素的敏感性，以及对环丙沙星、司帕沙星和左氧氟沙星的最低抑菌浓度。对gyrA基因中的氟喹诺酮耐药决定区（QRDR）进行了测序和分析。结果显示，3株菌株对异烟肼、利福平和/或链霉素耐药。所有菌株均对环丙沙星、左氧氟沙星和司帕沙星敏感。然而，在分离株中发现了独特的gyrA基因变异。一种菌株的 QRDR 存在突变（T73A）。此外，在13个菌株（81.25%）中观察到密码子88处的SerThr替换，所有这些菌株都对氟喹诺酮类药物敏感。表明本研究中观察到的gyrA变异可能为今后氟喹诺酮类药物治疗耐药结核分枝杆菌感染的研究提供有用的流行病学信息。完整的核苷酸序列已存入 GenBank 数据库，登录号为 AF400983。
The fluoroquinolone susceptibility and molecular epidemiology of Mycobacterium tuberculosis strains isolated from 16 pulmonary tuberculosis patients at the University Teaching Hospital Chest Clinic, Lusaka, Zambia, were investigated [date not given]. The susceptibility of the isolates to isoniazid, rifampicin, ethambutol and streptomycin, and the minimum inhibitory concentrations to ciprofloxacin, sparfloxacin and levofloxacin were determined. The fluoroquinolone resistance-determining region (QRDR) in gyrA gene was sequenced and analysed. The results showed that 3 strains were resistant to isoniazid, rifampicin and/or streptomycin. All strains were susceptible to ciprofloxacin, levofloxacin and sparfloxacin. However, a unique gyrA gene variation was identified in the isolates. One strain had a mutation (T73A) in QRDR. In addition, a SerThr substitution at codon 88 was observed in 13 strains (81.25%), all of which were susceptible to fluoroquinolones. It is suggested that the gyrA variation observed in this study may provide useful epidemiological information for future studies on fluoroquinolone therapy of resistant M. tuberculosis infection. The complete nucleotide sequence has been deposited in GenBank database under accession number AF400983.