A TRPV family ion channel required for hearing in Drosophila
A TRPV family ion channel required for hearing in Drosophila
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DOI：
10.1038/nature01733
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发表时间：
2003-07-03
期刊：
影响因子：
64.8
通讯作者：
Kim, C
中科院分区：
文献类型：
--
作者：
Kim, J;Chung, YD;Kim, C
许多类型的昆虫耳朵共享一个共同的感觉元件，弦音器官，其中声音诱导的触角或鼓膜振动被传递到纤毛感觉神经元并转换为受体电位（1，2）。然而，弦音神经元或任何听觉系统中的转导离子通道的分子身份仍然未知（3，4）。NOMPC（瞬时受体电位（TRP）超家族离子通道）突变的果蝇在触觉刚毛中缺乏受体电位和电流（5，6），但保留了大部分触角声音诱发反应（7），这表明不同的通道是弦音器官中的主要换能器。在这里，我们描述了果蝇南充（南）蛋白，离子通道亚基类似香草素受体相关（TRPV）通道的TRP超家族。Nan介导培养细胞中低渗透激活的钙内流和阳离子电流。它在体内仅在弦音神经元中表达，并定位于它们的感觉纤毛。在缺乏Nan的突变体中完全不存在听觉声音诱发电位，这表明它是弦音机械换能器的重要组成部分。
The many types of insect ear share a common sensory element, the chordotonal organ, in which sound-induced antennal or tympanal vibrations are transmitted to ciliated sensory neurons and transduced to receptor potentials(1,2). However, the molecular identity of the transducing ion channels in chordotonal neurons, or in any auditory system, is still unknown(3,4). Drosophila that are mutant for NOMPC, a transient receptor potential (TRP) superfamily ion channel, lack receptor potentials and currents in tactile bristles(5,6) but retain most of the antennal sound-evoked response(7), suggesting that a different channel is the primary transducer in chordotonal organs. Here we describe the Drosophila Nanchung (Nan) protein, an ion channel subunit similar to vanilloid-receptor-related (TRPV) channels of the TRP superfamily. Nan mediates hypo-osmotically activated calcium influx and cation currents in cultured cells. It is expressed in vivo exclusively in chordotonal neurons and is localized to their sensory cilia. Antennal sound-evoked potentials are completely absent in mutants lacking Nan, showing that it is an essential component of the chordotonal mechanotransducer.