Effects of grafting on the cadmium accumulation characteristics of the potential Cd-hyperaccumulator Solanum photeinocarpum
Effects of grafting on the cadmium accumulation characteristics of the potential Cd-hyperaccumulator Solanum photeinocarpum
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DOI：
10.1007/s10661-015-5084-3
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发表时间：
2016-01
影响因子：
3
通讯作者：
Lijin Lin;Daiyu Yang;Xun Wang;M. Liao;Zhihui Wang;X. Lv;Fuyi Tang;D. Liang;Hui Xia;Y. Lai;Yi Tang
中科院分区：
文献类型：
--
作者：
Lijin Lin;Daiyu Yang;Xun Wang;M. Liao;Zhihui Wang;X. Lv;Fuyi Tang;D. Liang;Hui Xia;Y. Lai;Yi Tang
研究了镉胁迫下嫁接对潜在镉超积累植物龙葵镉积累特性的影响。试验设不接、自根嫁接、自根嫁接四种处理。采用光果蓝实生苗（SG），通过光果蓝的两个不同发育时期进行自根嫁接。光果马铃薯实生苗（DG）和野生马铃薯（PG）砧木嫁接。SG和DG降低了根、接穗茎、叶、全苗和全株的生物量，但增加了砧木茎的生物量，而只有PG增加了根和全株的生物量。SG和DG处理提高了S.与UG相比，PG降低了Cd含量。采用全株提取法研究了S.光果类植物表现为DG > SG > UG > PG。此外，SG和DG的抗氧化酶活性比UG提高，而PG的抗氧化酶活性比UG降低。嫁接增加了DNA甲基化水平，改变了S.与UG相比，因此，SG和DG可以增加光果紫穗槐对Cd的积累，可用于Cd污染土壤的植物修复。
The effects of grafting on the cadmium (Cd) accumulation characteristics of the potential Cd-hyperaccumulatorSolanum photeinocarpumwere studied under Cd stress in our experiment. Four treatments were used in the experiment: ungrafted (UG), self-rooted grafting by the sameS. photeinocarpumseedling (SG), self-rooted grafting by two different development stages ofS. photeinocarpumseedlings (DG), and grafting on the rootstock of wild potato (PG). SG and DG decreased the root, scion stem, leaf, whole shoot, and whole plant biomasses compared with UG, but increased the rootstock stem biomass, while only PG increased the root and whole plant biomasses. SG and DG increased the Cd contents in the different organs ofS. photeinocarpumcompared with UG, while PG decreased the Cd content compared with UG. The Cd extraction by the whole plant ofS. photeinocarpumwas ranked as DG > SG > UG > PG. Additionally, the antioxidant enzyme activities in SG and DG were enhanced compared with UG, while that of PG was reduced compared with UG. The grafting increased the DNA methylation levels and changed the methylation patterns ofS. photeinocarpumcompared with UG. Therefore, SG and DG can increase the Cd accumulation inS. photeinocarpum, which can be used for the phytoremediation of Cd-contaminated soil.