Microfluidic valve with zero dead volume and negligible back-flow for droplets handling
Microfluidic valve with zero dead volume and negligible back-flow for droplets handling
复制标题
DOI：
10.1016/j.snb.2017.12.002
复制
发表时间：
2018-04-01
影响因子：
8.4
通讯作者：
Descroix, S.
中科院分区：
文献类型：
--
作者：
Ferraro, D.;Serra, M.;Descroix, S.
液滴微流控技术允许在微流控设备中实施几种生化和生物分析方案。然而，这种集成通常需要使用微流体阀，这允许控制相同通道网络上的不同操作。遗憾的是，商业上可用的阀门几乎完全适用于单相流，到目前为止，文献中只提出了一种与多相系统兼容的阀门系统。我们在这里介绍一种新的夹管阀，它可以承受高压力(测试高达4巴)，允许液滴操纵，并结合了传统夹管阀的优点，没有死气量，同时使产生的回流可以忽略不计(小于2 NL)。在数值模拟的基础上，结合PDMS软光刻和3D打印技术，采用优化的微细加工策略进行了阀门的构思和设计。这些阀门已经过机械稳定性测试，并证明了它们能够让液滴在没有任何扰动的情况下通过它们。最后，在驱动过程中产生的非常小的回流体积已被用于液滴微流体的各种应用，如液滴处理、按需生成液滴和分选。(C)2017爱思唯尔B.V.保留所有权利。
Droplet microfluidics allows the implementation of several biochemical and bioanalytical protocols in microfluidic devices. However, this integration typically requires the use of microfluidic valves, which allow the control of different operations on the same channels network. Unfortunately, the valves commercially available are almost exclusively suitable for-single phase flow, and only one valve system compatible with multi-phase systems has been presented so far in the literature. We present here anew pinch-like valve that withstands high pressures (tested up to 4 bar), allows droplet manipulation and combines the advantages of conventional pinch valves regarding the absence of dead volume, while making negligible the backflow generated (less than 2 nL). The conception and design of the presentedvalve, based on numerical simulations, is performed by an optimized microfabrication strategy, combining PDMS soft-lithography and 3D printing technologies. The valves had been tested regarding their mechanical stability and proving their capability of allowing droplets passing through them, without any perturbation. Finally, the very small back-flow volume generated during the actuation, has been exploited for various applications in droplets microfluidics, like droplet handling, droplet-on-demand generation and sorting. (C) 2017 Elsevier B.V. All rights reserved.