Monocyte dependence of pokeweed mitogen-induced differentiation of immunoglobulin-secreting cells from human peripheral blood mononuclear cells.
Monocyte dependence of pokeweed mitogen-induced differentiation of immunoglobulin-secreting cells from human peripheral blood mononuclear cells.
复制标题
美洲商陆有丝分裂原诱导的免疫球蛋白分泌细胞与人外周血单核细胞的分化的单核细胞依赖性。
作者：
S. Rosenberg;P. Lipsky
人外周血单个核细胞（PBM）在去除贴壁细胞（AC）后，对美洲商陆有丝分裂原（PWM）产生免疫球蛋白分泌细胞（ISC）的能力丧失。非贴壁细胞（NAC）的反应性降低不能归因于细胞死亡，改变PWM剂量反应特性，或产生反应所需的孵育时间的变化。补充自体或同源AC，但不是2-巯基乙醇，恢复NAC的能力，产生ISC PWM刺激后。根据标准标准，发现AC含有85至90%的单核细胞。此外，单核细胞，而不是少数淋巴细胞污染的AC负责恢复PWM响应NAC。PW M诱导的NAC DNA合成也明显低于PBM。再次，补充单核细胞恢复了对NAC的反应性。PWM诱导的增殖和ISC的产生的单核细胞依赖性是最明显的，当采用的文化条件，限制细胞间的相互作用。
Human peripheral blood mononuclear cells (PBM) lost the capacity to generate immunoglobulin-secreting cells (ISC) in response to pokeweed mitogen (PWM) when depleted of adherent cells (AC). The diminished responsiveness of the nonadherent cells (NAC) could not be ascribed to cell death, altered PWM dose response characteristics, or a change in the length of incubation required to generate a response. Supplementation with autologous or homologous AC, but not 2-mercaptoethanol, restored the capacity of NAC to generate ISC after PWM stimulation. By standard criteria AC were found to contain 85 to 90% monocytes. Furthermore, the monocytes and not the few lymphocytes contaminating the AC were responsible for restoring PWM responsiveness to the NAC. PWM-induced DNA synthesis of NAC also was markedly reduced compared to PBM. Again, supplementation with monocytes restored responsiveness to NAC. The monocyte dependence of PWM-induced proliferation and generation of ISC was most apparent when cultural conditions were employed that limited cell-to-cell interaction.