A novel model of polyhydramnios: amniotic fluid volume is increased in aquaporin 1 knockout mice
A novel model of polyhydramnios: amniotic fluid volume is increased in aquaporin 1 knockout mice
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DOI：
10.1016/j.ajog.2005.02.046
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发表时间：
2005-06-01
影响因子：
9.8
通讯作者：
Taylor, RN
中科院分区：
文献类型：
--
作者：
Mann, SE;Ricke, EA;Taylor, RN
目的：为了检验水通道蛋白1基因敲除小鼠羊水量增加的假设，研究设计：如前所述，通过靶向基因破坏产生水通道蛋白1蛋白缺陷的转基因小鼠。在进行剖腹产后，从子宫中取出完整的单个孕囊并称重。测定羊水量、渗透压、胎儿和胎盘重量。数据进行了分析，通过单因素方差分析的行列，邓恩事后检验被用来分析显着trends.Results：分析16窝显示35野生型，52杂合子，和33水通道蛋白1基因敲除小鼠。基因敲除小鼠的羊水量更大，羊水渗透压比野生型和杂合子小鼠低。有胎儿或胎盘重量组之间没有显着差异。结论：水通道蛋白1无效胎儿产生更大体积的更稀的羊水。我们的研究结果表明，水通道蛋白1在胎膜水通道可能有助于羊水量的调节。我们推测，特发性羊水过多可能与人类胎膜水通道蛋白1通道缺乏有关。转基因水通道蛋白1基因敲除小鼠提供了一种独特的羊水过多动物。(c)2005年爱思唯尔公司All rights reserved.
Objective: To test the hypothesis that amniotic fluid Volume is increased in aquaporin 1 knockout mice.Study design: Transgenic mice deficient in aquaporin 1 protein were generated by targeted gene disruption, as described previously. After a cesarean section was performed, intact, individual gestational sacs were removed from the uterus and weighed. Amniotic fluid volume, osmolality, and fetal and placental weights were determined. Data were analyzed by a 1-way analysis of variance for ranks; Dunn's post hoc test was used to analyze significant trends.Results: Analysis of 16 litters showed 35 wild-type, 52 heterozygote, and 33 aquaporin 1 knockout mice. The knockout mice had a greater volume of amniotic fluid and lower amniotic fluid osmolality than their wild-type and heterozygote Counterparts. There were no significant differences in fetal or placental weights among the groups.Conclusions: Aquaporin 1 null fetuses produce a greater Volume of more dilute amniotic fluid. our findings show that aquaporin 1 water channels in fetal membranes may contribute to amniotic fluid volume regulation. We speculate that idiopathic polyhydramnios may be associated with a deficiency of aquaporin 1 channels in human fetal membranes. Transgenic aquaporin 1 knockout mice provide a unique animal of polyhydramnios. (c) 2005 Elsevier Inc. All rights reserved.