Differentiation of immature DCs into endothelial-like cells in human esophageal carcinoma tissue homogenates
Differentiation of immature DCs into endothelial-like cells in human esophageal carcinoma tissue homogenates
复制标题
人食管癌组织匀浆中未成熟DC分化为内皮样细胞
DOI：
10.3892/or.2013.2491
复制
发表时间：
2013-08-01
期刊：
影响因子：
4.2
通讯作者：
Dong, Ziming
中科院分区：
文献类型：
--
作者：
Lu, Jing;Bai, Ruihua;Dong, Ziming
We previously reported endothelial-like differentiation (ELD) of immature dendritic cells (iDCs) in the microenvironment derived from EC9706 human esophageal squamous cell carcinoma conditioned medium (CM). However, the CM is far different from the esophageal carcinoma tissue of patients. In addition, the potential role of pen-esophageal carcinoma in the ELD of iDCs is also unknown. In the present study, we showed that the tumor microenvironment derived from esophageal carcinoma homogenate promoted iDCs to differentiate from the DC pathway toward endothelial cells, while the pen-esophageal carcinoma homogenate did not have this function. During the course of ELD, ERK signaling pathway and CREB were activated. Blocking MEK, both the phosphorylation of ERK and CREB, and the ELD of iDCs were inhibited. These data suggest that esophageal carcinoma tissue, not pen-esophageal carcinoma tissue, can drive iDCs to differentiate into endothelial-like cells, instead of differentiation into mature DCs, thereby losing the ability of antigen presentation.
