In vivo analysis of mtDNA replication defects in yeast
In vivo analysis of mtDNA replication defects in yeast
复制标题
DOI：
10.1016/j.ymeth.2010.02.023
复制
发表时间：
2010-08-01
期刊：
影响因子：
4.8
通讯作者：
Foury, Francoise
中科院分区：
文献类型：
--
作者：
Baruffini, Enrico;Ferrero, Iliana;Foury, Francoise
酵母酿酒酵母具有在线粒体DNA的大缺失或完全缺失（小突变体）下存活的能力，因此在过去几年中，它已被用作研究由参与线粒体DNA复制的基因中的突变产生的线粒体DNA（mtDNA）维持缺陷的模型系统。在本文中，我们描述的方法，以获得菌株窝藏突变的核基因的mtDNA的完整性，测量的频率和性质的娇小的突变体，估计点突变的频率在mtDNA和确定是否核突变是隐性或显性的，在后一种情况下，显性的种类。(C)2010年由Elsevier Inc.出版
The yeast Saccharomyces cerevisiae has the capacity to survive large deletions or total loss of mtDNA (petite mutants), and thus in the last few years it has been used as a model system to study defects in mitochondrial DNA (mtDNA) maintenance produced by mutations in genes involved in mtDNA replication. In this paper we describe methods to obtain strains harboring mutations in nuclear genes essential for the integrity of mtDNA, to measure the frequency and the nature of petite mutants, to estimate the point mutation frequency in mtDNA and to determine whether a nuclear mutation is recessive or dominant and, in the latter case, the kind of dominance. (C) 2010 Published by Elsevier Inc.