Ligand binding kinetics of the quorum sensing regulator PqsR.
Ligand binding kinetics of the quorum sensing regulator PqsR.
复制标题
群体感应调节剂 PqsR 的配体结合动力学。
作者：
Welch M
铜绿假单胞菌喹诺酮信号（PQS）是一种群体感应分子，在调节该菌的毒力中起重要作用。我们纯化了PQS受体PqsRLBD的配体结合结构域，并利用Fo Prster共振能量转移荧光法和动力学模型对配体结合进行了体外表征。PQS与（PqsRLBD）2二聚体中配体结合位点结合的解离常数为1.2 ± 0.3 μM。我们发现两个配体分子与二聚体的连续结合没有协同性。
ThePseudomonas aeruginosaquinolone signal (PQS) is a quorum sensing molecule that plays an important role in regulating the virulence of this organism. We have purified the ligand binding domain of the receptor PqsRLBDfor PQS and have used Förster resonance energy transfer fluorimetry and kinetic modeling to characterize the ligand bindingin vitro. The dissociation constant for binding of PQS to a ligand binding site in (PqsRLBD)2dimers was determined to be 1.2 ± 0.3 μM. We found no cooperativity in the consecutive binding of two ligand molecules to the dimer.
DOI：
10.1073/pnas.92.5.1490
发表时间：
1995-02-28
影响因子：
11.1
作者：
PEARSON, JP;PASSADOR, L;GREENBERG, EP
通讯作者：
GREENBERG, EP
DOI：
10.1073/pnas.96.20.11229
发表时间：
1999-09-28
影响因子：
11.1
作者：
Pesci, EC;Milbank, JBJ;Iglewski, BH
通讯作者：
Iglewski, BH