Preliminary Investigation of Microbial Community in Wastewater and Surface Waters in Sri Lanka and the Philippines
Preliminary Investigation of Microbial Community in Wastewater and Surface Waters in Sri Lanka and the Philippines
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DOI：
10.3389/frwa.2021.730124
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发表时间：
2021-12
期刊：
影响因子：
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通讯作者：
A. F. Cruz;R. Wijesekara;K. Jinadasa;B. Gonzales;T. Ohura;K. S. Guruge
中科院分区：
文献类型：
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作者：
A. F. Cruz;R. Wijesekara;K. Jinadasa;B. Gonzales;T. Ohura;K. S. Guruge
本研究通过16S rRNA基因扩增序列分析，研究了斯里兰卡和菲律宾的医院、商业性和非商业性养鱼场所、污水污水以及包括海水和淡水在内的地表水中经处理和未经处理的废水中细菌群落的组成和丰度。在斯里兰卡的医院废水中发现了大量的藻类，而在斯里兰卡的鱼类养殖场和污水渠中发现了典型的蓝藻和酸性细菌。菲律宾的Shannon-Weaver指数(Sw)和可操作分类单位数(OTUS)高于斯里兰卡。大学非商业性鱼塘和污水中的细菌丰富度高于医院废水。此外，未经处理的医院废水中细菌的丰富度高于处理后的废水。这些结果表明，不同类型水体的细菌群落存在差异，尤其是受其来源的影响。
In this study, the composition and richness of bacterial communities in treated and untreated wastewater from hospitals, commercial, and non-commercial fish farming sites, sewage effluents, and surface waters, which included seawater and fresh water in Sri Lanka and the Philippines, were investigated through 16S rRNA gene amplicon sequence analysis. Firmicutes were found predominantly in Sri Lankan hospital wastewaters, while Cyanobacteria and Acidobacteria were typically detected in fish culture sites and the waste canal in Sri Lanka, respectively. The Shannon–Weaver index (SW) and number of Operational Taxonomic Units (OTUs) were higher in the Philippines than in Sri Lanka. The bacterial richness in the university non-commercial fish pond and sewage effluent displayed greater than that in hospital wastewaters. In addition, the bacterial richness was higher in the untreated wastewater compared to that in the treated wastewater in hospitals. These results indicate the differences among water types in terms of bacterial community, especially influenced by their source.