EXPRESSION OF BOTH TYPES OF HUMAN INTERLEUKIN-8 RECEPTORS ON MATURE NEUTROPHILS, MONOCYTES, AND NATURAL-KILLER-CELLS
EXPRESSION OF BOTH TYPES OF HUMAN INTERLEUKIN-8 RECEPTORS ON MATURE NEUTROPHILS, MONOCYTES, AND NATURAL-KILLER-CELLS
复制标题
DOI：
10.1002/jlb.57.1.180
复制
发表时间：
1995-01-01
影响因子：
5.5
通讯作者：
MUKAIDA, N
中科院分区：
文献类型：
--
作者：
MOROHASHI, H;MIYAWAKI, T;MUKAIDA, N
基因克隆揭示了两种相关但不同类型的人白介素8(IL-8)受体，即I型(A型)和II型(B型)。将谷胱甘肽-S转移酶与各型IL-8受体的NH_2末端结构域融合免疫兔，制备了特异性识别各型IL-8受体NH_2末端结构域的多克隆抗体。人外周血白细胞的免疫荧光分析表明，除嗜酸性粒细胞外，成熟的粒细胞表达这两种类型的IL-8受体。外周血中大多数单核细胞和CD16(+)自然杀伤(NK)细胞同时表达两种抗体，而外周血中CD3(+)T、CD20(+)B细胞和脐带血CD34(+)细胞均不表达。这些结果表明，两种类型的人IL-8受体在成熟的粒细胞、单核细胞和CD16(+)NK细胞中协同和选择性地表达。
cDNA cloning revealed the presence of two related but distinct types of human interleukin-8 (IL-8) receptors, type I (type A) and type II (type B). By immunizing rabbits with glutathione-S-transferase fused with the NH2-terminal domain of each type of IL-8 receptor, we prepared polyclonal antibodies that specifically recognized the NH2-terminal domain of each type of IL-8 receptor. Immunofluorescence analysis of human peripheral blood leukocytes demonstrated that mature granulocytes except eosinophils express both types of IL-8 receptors. A majority of monocytes and CD16(+) natural killer (NK) cells in peripheral blood were stained with both antibodies, whereas CD3(+) T or CD20(+) B lymphocytes in peripheral blood or CD34(+) cells in cord blood were not stained with either antibody. These results suggest that both types of human IL-8 receptors were coordinately and selectively expressed in mature granulocytes, monocytes, and CD16(+) NK cells.