Differential Recognition of Nanoparticle Protein Corona and Modified Low-Density Lipoprotein by Macrophage Receptor with Collagenous Structure
Differential Recognition of Nanoparticle Protein Corona and Modified Low-Density Lipoprotein by Macrophage Receptor with Collagenous Structure
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DOI：
10.1021/acsnano.8b02014
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发表时间：
2018-05-01
期刊：
影响因子：
17.1
通讯作者：
Yan, Yan
中科院分区：
文献类型：
--
作者：
Lara, Sandra;Perez-Potti, Andre;Yan, Yan
关键的实际挑战，如了解在纳米级的免疫学过程和控制的目标和积累的纳米物体在体内，现在进一步刺激努力，以巩固纳米级的现象学知识与更多的机制和分子的洞察力。因此，关于什么构成纳米级生物身份的问题继续发展。当然，与复杂的生物环境接触的纳米颗粒会产生生物特性，与原始的纳米材料不同，现在被称为“生物分子冠”。然而，这种表面吸附的生物分子层在某些情况下可能导致不同形式的受体-颗粒相互作用，这些相互作用仅从表面吸附的生物分子的身份来看是不明显的，并且难以通过当前的物理化学方法预测或检测。在这里，我们表明，清道夫受体可以识别复杂的尚未确定的生物分子表面层图案，即使目前没有物理化学分析能够做到这一点。例如，生物环境中的荧光标记的SiO2纳米颗粒被甚至致密生物介质（人血清）中的具有胶原结构的巨噬细胞受体（MARCO）强烈识别，显然使用大多数MARCO的已知配体（例如，LPS，修饰的LDL）不能竞争。这样的观察可能表明，如果人们希望解开生物分子识别如何驱动纳米生物领域的结果，需要更加强调纳米级受体冠和其他生物分子相互作用的研究。
Key practical challenges such as understanding the immunological processes at the nanoscale and controlling the targeting and accumulation of nano-objects in vivo now further stimulate efforts to underpin phenomenological knowledge of the nanoscale with more mechanistic and molecular insight. Thus, the question as to what constitutes nanoscale biological identity continues to evolve. Certainly nanoparticles in contact with a complex biological milieu develop a biological identity, differing from the original nanomaterial, now referred to as the "biomolecular corona". However, this surface-adsorbed layer of biomolecules may in some circumstance lead to different forms of receptor-particle interactions not evident only from the identity of the surface-adsorbed biomolecules and hard to predict or detect by current physicochemical methods. Here we show that scavenger receptors may recognize complex as yet unidentified biomolecular surface layer motifs, even when no current physicochemical analysis is capable of doing so. For instance, fluorescently labeled SiO2 nanoparticles in a biological milieu are strongly recognized by the macrophage receptor with collagenous structure (MARCO) in even dense biological media (human serum) apparently using a form of binding with which most of the MARCO's known ligands (e.g., LPS, modified LDL) fail to compete. Such observations may suggest the need for a much stronger emphasis on nanoscale receptor-corona and other biomolecular interaction studies if one wishes to unravel how biomolecular recognition drives outcomes in the nanoscale biological domain.