Rat p67 GBP is induced by interferon-gamma and isoprenoid-modified in macrophages.
Rat p67 GBP is induced by interferon-gamma and isoprenoid-modified in macrophages.
复制标题
大鼠 p67 GBP 由巨噬细胞中的干扰素 γ 和类异戊二烯修饰诱导。
DOI：
10.1006/bbrc.1996.1060
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Maki,RA
中科院分区：
文献类型：
--
作者：
Vestal,DJ;Buss,JE;Kelner,GS;Maciejewski,D;Asundi,VK;Maki,RA
鸟苷酸结合蛋白（GBP）是干扰素诱导的GTP结合蛋白家族，包括大鼠p67。我们在这里报告，大鼠p67，其中干扰素的调节以前没有被证明，是由IFN-γ和LPS诱导培养的骨髓源性巨噬细胞和小胶质细胞。巨噬细胞中大鼠p67的基础水平较低，但在用IFN-γ或LPS处理细胞后2至4小时之间急剧增加。然后在接下来的24小时内保持升高。大鼠p67是类异戊二烯修饰的。在从原代IFN-γ激活的巨噬细胞中分离的p67中以及当p67基因转染COS细胞时检测到类异戊二烯修饰。这是第一次证明GBP的体内异戊二烯化。大鼠p67的干扰素调节和异戊烯化指向这种蛋白质在活化的巨噬细胞和小胶质细胞的功能中是重要的。
The guanylate binding proteins, GBPs, are a family of interferon-induced GTP-binding proteins that include the rat p67. We report here that rat p67, for which interferon regulation had not previously been demonstrated, is induced by IFN-γ and also by LPS in both cultured bone marrow-derived macrophages and microglia. The basal level of rat p67 in macrophages is low but increases dramatically between 2 and 4 hours after treating cells with either IFN-γ or LPS. It then remains elevated over the next 24 hours. Rat p67 is isoprenoid modified. The isoprenoid modification was detected in p67 isolated both from primary IFN-γ-activated macrophages and when the gene for p67 was transfected into COS cells. This is the first demonstration ofin vivoprenylation of a GBP. The interferon regulation and prenylation of rat p67 point toward this protein being significant in the functions of both activated macrophages and microglia.