DENDRITIC COSTRATIFICATION OF ON AND ON-OFF DIRECTIONALLY SELECTIVE GANGLION-CELLS WITH STARBURST AMACRINE CELLS IN RABBIT RETINA
DENDRITIC COSTRATIFICATION OF ON AND ON-OFF DIRECTIONALLY SELECTIVE GANGLION-CELLS WITH STARBURST AMACRINE CELLS IN RABBIT RETINA
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DOI：
10.1002/cne.903240303
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发表时间：
1992-10-15
影响因子：
2.5
通讯作者：
FAMIGLIETTI, EV
中科院分区：
文献类型：
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作者：
FAMIGLIETTI, EV
视网膜神经节细胞的形态，树突状分支模式，和树突状分层进行了研究，在高尔基浸渍，整体安装准备的兔视网膜。在大量的形态类型中，已发现两种对应于其他研究中确定的ON和ON-OFF方向选择性（DS）神经节细胞的形态。这些细胞在前面的论文中已经根据它们的细胞体大小、树突区大小和分支模式进行了表征。在本文中，两种DS神经节细胞的树突分层的水平进行了比较。它们相互比较，也与III.1类细胞的例子，在前面的文件中定义的参考我们以前的研究。研究采用计算机辅助，三维重建的树突状树，以及对一对具有重叠树突树的ON DS和ON-OFF DS神经节细胞的分析表明，这两种类型的DS神经节细胞在亚板层B的中间（层4）部分共分层。证实了某些ONDS神经节细胞向a亚层内伸出少量树突的报道。对ON-OFF DS神经节细胞和星状无长突细胞与重叠的树突树的研究揭示了这两种细胞类型的精确共分层，以及许多点的星状终扣与ON-OFF DS神经节细胞树突在两个亚层的内丛状层（IPL）的紧密并列。这是由高分辨率光学显微镜和电子显微镜证实。因此，可以得出结论，ON DS也与B型星状（胆碱能）无长突细胞部分共分层，并且当偶尔树突上升到该水平时，显然也与A型星状无长突细胞部分共分层。两种DS神经节细胞的共层化与共享某些共同的输入（包括锥双极细胞输入）是一致的。共同分层与星爆无长突细胞同意的强大的药理作用后，ON和ON-OFF DS神经节细胞的胆碱能agonistermine.The主要差异的报道，在树突状分层的bisterialized ON-OFF DS神经节细胞和一般unisterialized ON DS神经节细胞的生理演示是一致的双亚层组织的ON和OFF途径的强脉冲光。偶尔分支的ON DS细胞在sublamina的问题进行了讨论的阈值为OFF的反应。ON和ON-OFF DS神经节细胞在亚板层B中分层的轻微差异以及两者在重叠区中缺乏树突束震颤被解释为意味着ON DS细胞比ON-OFF DS细胞从星爆/胆碱能神经元接收更少的输入。树突过程中的这些差异与输入的差异一致，特别是在选择性GABA能通路中，这可能解释了它们在两组首选方向和两个速度偏好范围中的显着生理差异。
The morphology, dendritic branching patterns, and dendritic stratification of retinal ganglion cells have been studied in Golgi-impregnated, whole-mount preparations of rabbit retina. Among a large number of morphological types identified, two have been found that correspond to the morphology of ON and ON-OFF directionally selective (DS) ganglion cells identified in other studies. These cells have been characterized in the preceeding paper in terms of their cell body size, dendritic field size, and branching pattern. In this paper, the two kinds of DS ganglion cell are compared in terms of their levels of dendritic stratification. They are compared with each other and also with examples of class III.1 cells, defined in the preceding paper with reference to our previous studies.Studies employing computer-aided, 3D reconstruction of dendritic trees, as well as analysis of a pair of ON DS and ON-OFF DS ganglion cells with overlapping dendritic trees show that the two types of DS ganglion cell partly co-stratify in the middle of sublamina b (stratum 4). The report that some ON DS ganglion cells extend a few dendrites into sublamina a is confirmed. The study of pairs of ON-OFF DS ganglion cells and starburst amacrine cells with overlapping dendritic trees reveals a precise co-stratification of these two cell types, and many points of close apposition of starburst boutons with ON-OFF DS ganglion cell dendrites in both sublaminae of the inner plexiform layer (IPL). This is confirmed by high-resolution light microscopy and by electron microscopy. It is possible to conclude, therefore, that ON DS are also partly co-stratified with type b starburst (cholinergic) amacrine cells, and are apparently also partly co-stratified with type a starburst amacrine cells, when occasional dendrites rise to that level. The co-stratification of the two kinds of DS ganglion cell is consistent with the sharing of some inputs in common, including some cone bipolar cell inputs. The co-stratification of both with starburst amacrine cells agrees with the physiological demonstration of the powerful pharmacological effects upon ON and ON-OFF DS ganglion cells reported for cholinergic agonists.The major difference in the dendritic stratification of bistratified ON-OFF DS ganglion cells and generally unistratified ON DS ganglion cells is consistent with the bisublaminar organization of ON and OFF pathways in the IPL. The problem of occasional branches of ON DS cells in sublamina a is discussed in terms of a threshold for OFF responses. The slight difference in stratification between ON and ON-OFF DS ganglion cells in sublamina b and lack of dendritic fasciculation of the two in the zone of overlap is interpreted to mean that ON DS cells receive less input from starburst/cholinergic neurons that ON-OFF DS cells. These differences in the course of dendrites are consistent with a difference in inputs, particularly in selective GABAergic pathways likely to account for their notable physiological differences in the two sets of preferred directions and in two ranges of velocity preference.