Reorganization of structural proteins in vascular smooth muscle cells grown in collagen gel and basement membrane matrices (Matrigel): a comparison with their in situ counterparts.
Reorganization of structural proteins in vascular smooth muscle cells grown in collagen gel and basement membrane matrices (Matrigel): a comparison with their in situ counterparts.
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在胶原凝胶和基底膜基质 (Matrigel) 中生长的血管平滑肌细胞中结构蛋白的重组：与原位对应物的比较。
DOI：
10.1006/jsbi.2001.4327
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发表时间：
2001
影响因子：
3
通讯作者：
J. Campbell
中科院分区：
文献类型：
--
作者：
J. Song;B. Rolfe;I. Hayward;G. Campbell;J. Campbell
当平滑肌细胞被酶从组织中分散时，它们失去了它们原始的细丝结构和细胞外基质包围。然后，它们重组其结构蛋白，以适应2-D生长环境时，接种到培养皿。本研究的目的是确定的表达和重组的结构蛋白在兔主动脉平滑肌细胞接种到3-D胶原凝胶和基质胶（基底膜基质）。结果表明，接种在两种凝胶中的平滑肌细胞逐渐将其结构蛋白重组成与其体内对应物相似的结构。同时，平滑肌特异性收缩蛋白（主要是平滑肌肌球蛋白重链-2）水平逐渐降低，β-非肌肉肌动蛋白水平增加，与细胞生长和细胞外基质成分无关。因此，在3-D细胞外基质培养和体内的平滑肌细胞具有类似的细丝结构，其中收缩蛋白如肌动蛋白、肌球蛋白和α-辅肌动蛋白被组织成纵向排列的“肌原纤维”，并且含有波形蛋白的中间细丝形成网状细胞骨架网络。然而，在体外重组的肌原纤维含有较少的平滑肌特异性和更多的非肌肉收缩蛋白。
When smooth muscle cells are enzyme-dispersed from tissues they lose their original filament architecture and extracellular matrix surrounds. They then reorganize their structural proteins to accommodate a 2-D growth environment when seeded onto culture dishes. The aim of the present study was to determine the expression and reorganization of the structural proteins in rabbit aortic smooth muscle cells seeded into 3-D collagen gel and Matrigel (a basement membrane matrix). It was shown that smooth muscle cells seeded in both gels gradually reorganize their structural proteins into an architecture similar to that of their in vivo counterparts. At the same time, a gradual decrease in levels of smooth muscle-specific contractile proteins (mainly smooth muscle myosin heavy chain-2) and an increase in beta-nonmuscle actin occur, independent of both cell growth and extracellular matrix components. Thus, smooth muscle cells in 3-D extracellular matrix culture and in vivo have a similar filament architecture in which the contractile proteins such as actin, myosin, and alpha-actinin are organized into longitudinally arranged "myofibrils" and the vimentin-containing intermediate filaments form a meshed cytoskeletal network. However, the myofibrils reorganized in vitro contain less smooth muscle-specific and more nonmuscle contractile proteins.
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影响因子：
33.6
作者：
G. Owens
通讯作者：
G. Owens
肌动蛋白亚型表达、细胞异质性和平滑肌的收缩功能。
DOI：
--
发表时间：
1997
期刊：
Canadian journal of physiology and pharmacology.
影响因子：
--
作者：
Drew,JS;Murphy,RA
通讯作者：
Murphy,RA
在基质胶上生长的血管平滑肌细胞。
DOI：
--
发表时间：
1994
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Li,X;Tsai,P;Wieder,ED;Kribben,A;VanPutten,V;Schrier,RW;Nemenoff,RA
通讯作者：
Nemenoff,RA
DOI：
10.1006/bbrc.2000.2769
发表时间：
2000-05-27
影响因子：
3.1
作者：
Morla, AO;Mogford, JE
通讯作者：
Mogford, JE