Cell swelling following recovery from acidification in C6 glioma cells: an in vitro model of postischemic brain edema
Cell swelling following recovery from acidification in C6 glioma cells: an in vitro model of postischemic brain edema
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C6 胶质瘤细胞酸化恢复后细胞肿胀：缺血后脑水肿的体外模型
DOI：
10.1016/0006-8993(87)91594-0
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发表时间：
1987
期刊：
影响因子：
2.9
通讯作者：
A. Klip
中科院分区：
文献类型：
--
作者：
Difat E. Jakubovicz;S. Grinstein;A. Klip
脑缺血的两个后果是细胞酸化和细胞毒性水肿。为了测试Na+/H+交换介导酸诱导的水肿的可能性，我们测量了使用弱电解质人工加载酸的C6胶质瘤细胞中的细胞质pH（pHi）和细胞体积变化。用2′，7 ′-双（2-羧乙基）-5，6-羧基荧光素荧光法测定pH值。用丙酸钠酸化后。pHi下降至6.74 ± 0.05（n= 25），然后恢复至接近生理值7.23 ± 0.02（n= 13）。细胞体积，测量电子尺寸，同时增加了约50%的丙酸钠溶液。pH恢复和细胞肿胀都是Na+依赖性的，阿米洛利敏感的，并且在pH < 6.0时受到抑制。这些结果表明，在体外：（1）细胞内酸化可导致细胞肿胀，（2）pH恢复和伴随的细胞肿胀可能是由Na+/H+交换介导的。这些机制可能与脑缺血后细胞毒性胶质细胞水肿有关。
Two consequences of cerebral ischemia are cell acidification and cytotoxic edema. To test the possibility that Na+/H+exchange mediates acid-induced edema, we measured cytoplasmic pH (pHi) and cell volume changes in C6 glioma cells that were artificially acid-loaded using weak electrolytes. pHiwas monitored fluorimetrically with 2′,7′-bis-(2-carboxyethyl)-5,6-carboxyfluorescein. Upon acidification with sodium propionate. pHidropped to 6.74 ± 0.05 (n= 25), and then recovered to levels near the physiological value of 7.23 ± 0.02 (n= 13). Cell volume, measured by electronic sizing, increased concomitantly by approximately 50% in sodium propionate solution. Both pHirecovery and cell swelling were Na+-dependent, amiloride-sensitive, and inhibited at pHo< 6.0. These results demonstrate that in vitro: (1) intracellular acidification can lead to cell swelling, and (2) pHirecovery and the concomitant cell swelling are likely mediated by Na+/H+exchange. These mechanisms may be related to postischemic cytotoxic glial edema.
影响因子：
--
作者：
B. Siesjö
通讯作者：
B. Siesjö
缺血时的脑代谢：治疗的神经化学基础。
影响因子：
9.8
作者：
Siesjö,BK;Wieloch,T
通讯作者：
Wieloch,T
DOI：
10.1016/s0021-9258(18)32287-7
发表时间：
1983-06
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
P. Aronson;M. A. Suhm;J. Nee
通讯作者：
P. Aronson;M. A. Suhm;J. Nee