Osteogenic differentiation of human mesenchymal stem cells in freeze-gelled chitosan/nano β-tricalcium phosphate porous scaffolds crosslinked with genipin.
Osteogenic differentiation of human mesenchymal stem cells in freeze-gelled chitosan/nano β-tricalcium phosphate porous scaffolds crosslinked with genipin.
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DOI：
10.1016/j.msec.2015.05.005
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发表时间：
2015-09
期刊：
影响因子：
--
通讯作者：
Jabbari E
中科院分区：
文献类型：
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作者：
Siddiqui N;Pramanik K;Jabbari E
研究京尼平（GN）交联壳聚糖/纳米β-磷酸三钙（CS/nano β-TCP）支架材料的材料特性及人骨髓间充质干细胞（hMSCs）的成骨分化能力，并与三聚磷酸盐（TPP）交联支架进行比较。以GN（CBG支架）和TPP（CBT支架）为交联剂，采用冷冻凝胶法制备多孔交联CS/纳米β-TCP支架。制备的CBT和CBG支架的特征在于相对于孔隙率，孔径，含水量，润湿性，抗压强度，质量损失，和hMSCs的成骨分化。所有支架显示出互连的蜂窝状微观结构。CBT和CBG支架的平均孔径、孔隙率、接触角和溶胀百分比之间存在显著差异。CBG支架的平均孔径大于CBT，孔隙率小于CBT，水接触角大于CBT，溶胀率小于CBT。在给定的交联剂浓度下，CBG和CBT支架的压缩模量和质量损失没有显著差异。在CBG支架中接种的hMSCs的代谢活性略高于CBT。此外，与CBT相比，CBG支架在成骨培养基中孵育21天后显示出略高的矿化程度。
The objective of this work was to investigate material properties and osteogenic differentiation of human mesenchymal stem cells (hMSCs) in genipin (GN) crosslinked chitosan/nano β-tricalcium phosphate (CS/nano β-TCP) scaffolds, and compare the results with tripolyphosphate (TPP) crosslinked scaffolds. Porous crosslinked CS/nano β-TCP scaffolds were produced by freeze-gelation using GN (CBG scaffold) and TPP (CBT scaffold) as crosslinkers. The prepared CBT and CBG scaffolds were characterized with respect to porosity, pore size, water content, wettability, compressive strength, mass loss, and osteogenic differentiation of hMSCs. All scaffolds displayed interconnected honeycomb-like microstructures. There was a significant difference between the average pore size, porosity, contact angle, and percent swelling of CBT and CBG scaffolds. The average pore size of CBG scaffolds was higher than CBT, the porosity of CBG was lower than CBT, the water contact angle of CBG was higher than CBT, and the percent swelling of CBG was lower than CBT. At a given crosslinker concentration, there was not a significant difference in compressive modulus and mass loss of CBG and CBT scaffolds. Metabolic activity of hMSCs seeded in CBG scaffolds was slightly higher than CBT. Furthermore, CBG scaffolds displayed slightly higher extent of mineralization after 21 days incubation in osteogenic medium compared to CBT.