ANAEROBIC DEGRADATION OF ETHYLBENZENE AND OTHER AROMATIC-HYDROCARBONS BY NEW DENITRIFYING BACTERIA
ANAEROBIC DEGRADATION OF ETHYLBENZENE AND OTHER AROMATIC-HYDROCARBONS BY NEW DENITRIFYING BACTERIA
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DOI：
10.1007/bf00381782
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发表时间：
1995-02-01
影响因子：
2.8
通讯作者：
WIDDEL, F
中科院分区：
文献类型：
--
作者：
RABUS, R;WIDDEL, F
研究了在硝酸盐存在下，侧链比甲苯长的烷基苯类化合物在淡水泥浆中的厌氧降解。在甲苯和正丙苯上分离到两株新的产酶菌株EbN 1和PbN 1。为了进行比较，两个进一步的微生物菌株，ToN 1和mXyN 1，分离自相同的泥浆与甲苯和间二甲苯，分别。16 S rDNA序列分析表明，新分离物与Thauera selenatis亲缘关系较近。菌株表现出不同的降解烷基苯的能力。除甲苯外，菌株EbN 1还利用甲苯，但不利用丙苯。相比之下，丙苯降解菌株PbN 1不生长在甲苯上，但能够利用甲苯。菌株ToN 1使用甲苯作为唯一的烃底物，而菌株mXyN 1利用甲苯和间二甲苯。降解平衡的测量表明，菌株EbN 1完全将CO2氧化为CO2。菌株EbN 1和PbN 1的其他特征底物是1-苯基乙醇和苯乙酮。与其他分离株相比，菌株mXyN 1在苯甲醇上不生长。苯甲醇（也称为间甲基苯甲醇）甚至是菌株mXyN 1利用甲苯和间二甲苯的特异性抑制剂。没有一种菌株能够在以氧作为电子受体的任何烷基苯上生长。然而，极性芳香族化合物，如苯甲酸盐，利用在好氧和缺氧条件下。所有四种分离株均在原油上厌氧生长。在菌株ToN 1生长后的原油的气相色谱分析揭示了甲苯的特定消耗。
Anaerobic degradation of alkylbenzenes with side chains longer than that of toluene was studied in freshwater mud samples in the presence of nitrate. Two new denitrifying strains, EbN1 and PbN1, were isolated on ethylbenzene and n-propylbenzene, respectively. For comparison, two further denitrifying strains, ToN1 and mXyN1, were isolated from the same mud with toluene and m-xylene, respectively. Sequencing of 16S rDNA revealed a close relationship of the new isolates to Thauera selenatis. The strains exhibited different specific capacities for degradation of alkylbenzenes. In addition to ethylbenzene, strain EbN1 utilized toluene, but not propylbenzene. In contrast, propylbenzene-degrading strain PbN1 did not grow on toluene, but was able to utilize ethylbenzene. Strain ToN1 used toluene as the only hydrocarbon substrate, whereas strain mXyN1 utilized both toluene and m-xylene. Measurement of the degradation balance demonstrated complete oxidation of ethylbenzene to CO2 by strain EbN1. Further characteristic substrates of strains EbN1 and PbN1 were 1-phenylethanol and acetophenone. In contrast to the other isolates, strain mXyN1 did not grow on benzyl alcohol. Benzyl alcohol (also m-methylbenzyl alcohol) was even a specific inhibitor of toluene and m-xylene utilization by strain mXyN1. None of the strains was able to grow on any of the alkylbenzenes with oxygen as electron acceptor. However, polar aromatic compounds such as benzoate were utilized under both oxic and anoxic conditions. All four isolates grew anaerobically on crude oil. Gas chromatographic analysis of crude oil after growth of strain ToN1 revealed specific depletion of toluene.