High-throughput multiplexed autoantibody detection to screen type 1 diabetes and multiple autoimmune diseases simultaneously
High-throughput multiplexed autoantibody detection to screen type 1 diabetes and multiple autoimmune diseases simultaneously
复制标题
高通量多重自身抗体检测同时筛查1型糖尿病和多种自身免疫性疾病
DOI：
10.1016/j.ebiom.2019.08.036
复制
发表时间：
2019-09-01
期刊：
影响因子：
11.1
通讯作者：
Yu, Liping
中科院分区：
文献类型：
--
作者：
Gu, Yong;Zhao, Zhiyuan;Yu, Liping
背景：胰岛自身抗体（IAb）是评估临床1型糖尿病（T1 D）进展风险的最可靠生物标志物。目前在临床试验中使用的四种主要的生化定义的IAb对疾病预测同样重要。目前的IAb筛选方法采用放射结合法（Radio-binding assay，RBA）进行单克隆抗体的检测，这对于大规模的IAb筛选来说是费力且低效的。更重要的是，高达40%的T1 D患者有其他自身免疫性疾病，可以通过相关的自身抗体检测来识别。因此，需要同时筛查T1 D和其他自身免疫性疾病。方法：基于我们完善的电化学发光（ECL）测定平台，我们开发了一种多重ECL测定，其在一个单一孔中将7种单独的自身抗体测定组合在一起，以同时筛查T1 D和其他三种自身免疫性疾病，包括乳糜泻，自身免疫性甲状腺疾病和自身免疫性多腺体综合征-1（APS-1）。7-β ECL检测针对单抗体测量进行了广泛验证，包括标准RBA和单ECL检测。结果：7-β ECL检测与每个单ECL自身抗体检测和每个RBA都有良好的相关性。解释：多重ECL测定提供高灵敏度和疾病特异性，沿着高通量和低成本，用于在普通人群中大规模筛查T1 D和其他相关自身免疫疾病。(C)2019作者由爱思唯尔公司出版
Background: Islet autoantibodies (IAbs) are the most reliable biomarkers to assess risk of progression to clinical type 1 diabetes (T1D). There are four major biochemically defined IAbs currently used in clinical trials that are equally important for disease prediction. The current screening methods use a radio-binding assay (RBA) for single IAb measurement, which are laborious and inefficient for large-scale screening. More importantly, up to 40% of patients with T1D have other autoimmune conditions that can be identified through relevant autoantibody testing. Thus, there is a need to screen for T1D and other autoimmune diseases simultaneously.Methods: Based on our well-established electrochemiluminescence (ECL) assay platform, we developed a multiplexed ECL assay that combines 7 individual autoantibody assays together in one single well to simultaneously screen T1D, and three other autoimmune diseases including celiac disease, autoimmune thyroid disease and autoimmune poly-glandular syndrome-1 (APS-1). The 7-Plex ECL assay was extensively validated against single antibody measurements including a standard RBA and single ECL assay.Findings: The 7-Plex ECL assay was well correlated to each single ECL autoantibody assay and each RBA.Interpretation: The multiplexed ECL assay provides high sensitivity and disease specificity, along with high throughput and a low cost for large-scale screenings of T1D and other relevant autoimmune diseases in the general population. (C) 2019 The Authors. Published by Elsevier B.V.