Analysis of the Germinal Vesicle Requirement for the Activation of MPF in Maturation of Porcine Oocytes
Analysis of the Germinal Vesicle Requirement for the Activation of MPF in Maturation of Porcine Oocytes
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猪卵母细胞成熟过程中MPF激活的胚泡需求分析
DOI：
10.1274/jmor.16.130
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
H. Tojo
中科院分区：
文献类型：
--
作者：
K. Sugiura;K. Naito;H. Kagii;N. Iwamori;K. Yamanouchi;H. Tojo
研究了猪卵母细胞成熟过程中生发囊泡（GV）激活MPF的必要性。将猪卵泡卵母细胞去核，不破坏卵泡膜，对未培养和培养30 h的去核卵母细胞进行蛋白合成和MPF活性检测。去核卵母细胞的蛋白合成模式在培养前后30h发生了变化，这种变化与完整卵母细胞在GV破坏前后的变化完全相同。在积云封闭卵母细胞和剥落卵母细胞培养30 h后，MPF活性分别提高了约7倍和5倍。培养30 h后，去核卵母细胞的MPF活性与去核卵母细胞相同。这些结果表明，在猪卵母细胞成熟过程中，GV的存在并不是正常蛋白质合成所必需的，而通常认为的核-胞质相互作用、细胞周期蛋白B进入GV以及核质与细胞质的混合也不是MPF激活所必需的。
The necessity of the germinal vesicle (GV) for the activation of MPF during porcine oocyte maturation was investigated. Porcine follicular oocytes were enucleated without damaging the oolemma, and the enucleated oocytes without culture or cultured for 30 h were examined for their protein synthesis and MPF activities. The protein synthesis pattern in enucleated oocytes changed between before and after 30 h of culture, and this change was exactly the same as that of intact oocytes between before and after GV breakdown. The MPF activity increased about 7 and 5 times after 30 h of culture in cumulus enclosed and denuded oocytes, respectively. The activation of MPF was also observed in the enucleated oocytes after 30 h of culture and the MPF activity was the same level as that of denuded oocytes. These results indicate that the presence of GV is not required for normal protein synthesis during porcine oocyte maturation and that the generally suggested nucleus-cytoplasmic interactions, cyclin B movement into GV and the mixing of karyoplasm with cytoplasm, are not required for the MPF activation.
DOI：
10.1073/pnas.85.9.3009
发表时间：
1988-05-01
影响因子：
11.1
作者：
LOHKA, MJ;HAYES, MK;MALLER, JL
通讯作者：
MALLER, JL