Polygonatum sibiricum polysaccharides play anti-cancer effect through TLR4-MAPK/NF-κB signaling pathways
Polygonatum sibiricum polysaccharides play anti-cancer effect through TLR4-MAPK/NF-κB signaling pathways
复制标题
黄精多糖通过TLR4-MAPK/NF-kappa B信号通路发挥抗癌作用
DOI：
10.1016/j.ijbiomac.2018.01.070
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发表时间：
2018-05-01
影响因子：
8.2
通讯作者：
Bao, Yixi
中科院分区：
文献类型：
--
作者：
Long, Tingting;Liu, Zijing;Bao, Yixi
目的：目的探讨黄精多糖（PSP）的抗癌作用及其机制方法：将荷瘤小鼠随机分为生理盐水（NS）组、阿霉素（ADM）组、黄精多糖（PSP）组和脂多糖（LPS）组。用或不用TLR 4抑制剂或MyD 88抑制剂预处理RAW264.7细胞。采用RT-PCR和Western blot方法分别检测mRNA和蛋白表达水平。采用酶联免疫吸附试验（ELISA）和Griess反应检测细胞因子和NO水平。结果：PSP能明显抑制荷瘤小鼠的肿瘤生长，提高小鼠的脾指数、胸腺指数、细胞因子分泌量和CD 4 +/CD 8+淋巴细胞比值。与NS组相比，PSP组TLR 4-MAPK/NF-κ B信号通路关键节点（TRAM除外）的mRNA和蛋白表达均显著增加，NO和细胞因子水平也显著升高。PSP对TRAM无明显影响。结论：PSP对肺癌细胞的免疫增强作用是通过TLR 4-MAPK/NF-κ B信号通路介导的。(C)2018爱思唯尔B. V.保留所有权利。
Objective: To investigate the anti-cancer effect of Polygonatum sibiricum polysaccharides (PSP) and the underlying mechanism.Methods: Tumor-beating mice were randomly divided into normal saline (NS) group, adriamycin (ADM) group, PSP group and lipopolysaccharide (LPS) group. RAW264.7 cells were pre-treated with or without TLR4 inhibitor or MyD88 inhibitor. Quantitative RT-PCR and Western blot were performed to detect the mRNA and protein expressions, respectively. ELISA and Griess reaction was used to measure cytokines and NO levels. Flow cytometry was employed to examine T-lymphocyte subset and CCK-8 assay was used for cell viability.Results: The in vivo experiment found that PSP inhibited tumor growth and improved the spleen index, thymus index, the cytokines secretion and CD4(+)/CD8(+) lymphocytes ratio. Compared with the NS group, the mRNA and protein expressions of the critical nodes inTLR4-MAPK/NF-kappa B signaling pathways (except TRAM) significantly increased in PSP group, as well as the NO and cytokines levels. Nevertheless, PSP had no obvious effects on TRAM. Further analysis showed that PSP effects on the critical nodes in TLR4-MAPK/NF-kappa B signaling pathways were suppressed by inhibitor in vitro.Conclusion: The immunoenhancement effect of PSP against lung cancer is mediated by TLR4-MAPK/NF-kappa B signaling pathways. (C) 2018 Elsevier B.V. All rights reserved.