Reliable size determination of nanoparticles using dynamic light scattering method for in vitro toxicology assessment
Reliable size determination of nanoparticles using dynamic light scattering method for in vitro toxicology assessment
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DOI：
10.1016/j.tiv.2009.04.006
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发表时间：
2009-08-01
影响因子：
3.2
通讯作者：
Kinugasa, Shinichi
中科院分区：
文献类型：
--
作者：
Kato, Haruhisa;Suzuki, Mie;Kinugasa, Shinichi
动态光散射（DLS）在纳米粒子的毒性评估中被广泛用于粒径评估。然而，多种类型的DLS仪器和分析程序有时会给出不同的表观粒径，这使得理解毒性测定中粒径对粒子的依赖性变得复杂。在本研究中，我们利用DLS分析建立了一种二次纳米粒子粒径的评估方法。首先，我们建立了一种确定粒径并对不确定性进行适当评估的实用方法。这种提出的方法可以作为一种通用的毒性评估方案，使研究人员在纳米粒子粒径评估上达到一定程度的一致性。其次，由于粒子向细胞的传输模式对于理解体外纳米毒性至关重要，我们通过检测体外毒性评估过程中二次纳米粒子粒径和光散射强度的变化，研究了悬浮液中与粒子相关的过程。在本研究中，以二氧化钛纳米粒子悬浮液为例对这两点进行了研究。光散射强度平均直径（d(t)）为150 - 250 nm的二氧化钛二次粒子在具有适当不确定性的情况下被表征。发现其粒径与使用其他分析程序和仪器测定的值具有可比性。这表明d(t)可以作为毒性评估的一个有效粒径参数。此外，二氧化钛二次纳米粒子悬浮液分散良好，重力沉降缓慢，无团聚，扩散过程是粒子向细胞传输的主要模式。（C）2009爱思唯尔有限公司。保留所有权利。
Dynamic light scattering (OLS) is widely used for the evaluation of the particle size in the toxicity assessment of nanoparticles. However, the many types of DLS instruments and analytical procedures sometimes give different apparent sizes of particles and make it complicated to understand the size dependence on particles for the toxicity assay. In this study, we established an evaluation method of seconclary nanoparticle sizes using a DLS analysis. First, we established a practical method for determining size with an appropriate evaluation of uncertainties. This proposed method could be a universal protocol for toxicity assessment that would allow researchers to achieve some degree of concordance on the size of nanoparticles for an assessment. Second, we investigated the processes associated with particles in suspension by examining the changes in the size and the light scattering intensity of secondary nanoparticles during an in vitro toxicity assessment, since the transport mode of particles to cells is significant in understanding in vitro nano-toxicity. In this study, these two points were investigated on TiO2 nanoparticles suspension as an example. The secondary particles of TiO2 With a light scattering intensity-averaged diameter (d(t)) of 150-250 nm were characterized with appropriate uncertainties. The sizes were found to be comparable with values determined using other analytical procedures and other instruments. It is suggested that d(t) could be an effective size parameter for toxicity assessments. Furthermore, TiO2 secondary nanoparticle suspensions are well dispersed with slow gravity settling. no agglomeration, with the diffusion process as the primary transport mode of particles to cells. (C) 2009 Elsevier Ltd. All rights reserved.