Sus1, Sac3, and Thp1 mediate post-transcriptional tethering of active genes to the nuclear rim as well as to non-nascent mRNP
Sus1, Sac3, and Thp1 mediate post-transcriptional tethering of active genes to the nuclear rim as well as to non-nascent mRNP
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DOI：
10.1261/rna.764108
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发表时间：
2008-01-01
期刊：
影响因子：
4.5
通讯作者：
Belostotsky, Dmitry A.
中科院分区：
文献类型：
--
作者：
Chekanova, Julia A.;Abruzzi, Katharine C.;Belostotsky, Dmitry A.
mRNP生物合成途径中的错误可导致mRNA保留在离散的转录位点近端病灶中。这种RNA在转录关闭后很长一段时间内仍然被束缚在转录位点附近。在这里，我们确定Sus 1，Thp 1和Sac 3作为持续拴系这些病灶（点）到其同源基因所需的因素。我们还表明，以前激活的GAL基因与转录关闭后的核周边的长期协会同样依赖于Sac 3-Thp 1-Sus 1-Cdc 31复合物。我们认为，该复合物与核mRNP和mRNP属性影响关联的点限制mRNA与其基因的起源，以及在核周边的同源基因的转录后保留。这些发现表明mRNA-基因和基因-核外围束缚之间存在耦合。与其他最近的研究结果一起，这些观察结果也突出了核mRNP的重要性，以动员活性基因的核边缘。
Errors in the mRNP biogenesis pathway can lead to retention of mRNA in discrete, transcription-site-proximal foci. This RNA remains tethered adjacent to the transcription site long after transcriptional shutoff. Here we identify Sus1, Thp1, and Sac3 as factors required for the persistent tethering of such foci (dots) to their cognate genes. We also show that the prolonged association of previously activated GAL genes with the nuclear periphery after transcriptional shutoff is similarly dependent on the Sac3-Thp1-Sus1-Cdc31 complex. We suggest that the complex associates with nuclear mRNP and that mRNP properties influence the association of dot-confined mRNA with its gene of origin as well as the post-transcriptional retention of the cognate gene at the nuclear periphery. These findings indicate a coupling between the mRNA-to-gene and gene-to-nuclear periphery tethering. Taken together with other recent findings, these observations also highlight the importance of nuclear mRNP to the mobilization of active genes to the nuclear rim.