Single-Cell Protein Profiling by Microdroplet Barcoding and Next-Generation Sequencing.
Single-Cell Protein Profiling by Microdroplet Barcoding and Next-Generation Sequencing.
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DOI：
10.1007/978-1-0716-1771-7_7
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发表时间：
2022
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中科院分区：
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DNA barcoding of individual cells combined with next generation sequencing enables high-throughput parallel analysis of biomolecules at the single-cell level. Encoding protein identity with DNA barcoding of specific antibody binders achieves sequencing-based protein quantitation by converting protein signals into DNA signals. Here we describe how to prepare DNA-barcoded antibodies and connect protein identities to cellular identities using droplet microfluidics. This approach allows for multiplex single cell protein analysis compatible with single cell transcriptomic and mutational profiling methods.
