Detection of BK polyomavirus genotypes in healthy and HIV-positive children
Detection of BK polyomavirus genotypes in healthy and HIV-positive children
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DOI：
10.1023/a:1007371320999
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发表时间：
1997-09-01
影响因子：
13.6
通讯作者：
Degener, AM
中科院分区：
文献类型：
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作者：
DiTaranto, C;Pietropaolo, V;Degener, AM
收集了211名社区儿童（3-7岁）、33名HIV 1型感染儿童和56名HIV阴性儿童的尿液样本，并通过PCR分析BK病毒（BKV）DNA的存在。使用BKV结构区VP 1特异性引物进行PCR扩增。我们还调查了BKV亚型的分布通过限制性片段多态性分析（RFLP）。我们证实，在211名社区儿童中，BKV DNA阳性率为3.8%，亚型I的患病率较高。在HIV-1阳性的33名儿童中，我们在2份尿样（6%）中检测到BKV DNA，均属于亚型I。HIV阴性的病毒群对BKV DNA无阳性反应。结果证实，3-5岁儿童中BKV原发感染更频繁，感染HIV-1的住院儿童中患病率更高。最相关的调查结果是，在社区和艾滋病毒-1阳性儿童中，亚型I是最常见的。
Urine samples from 211 community children (3-7 years age), from 33 HIV type-1 infected children and from 56 HIV-negative children were collected and analyzed for the presence of BK virus (BKV) DNA by PCR. PCR amplifications were carried out using primers specific for the BKV structural region VP1. We also investigated the distribution of BKV subtypes by a restriction fragment polymorphism assay (RFLP). We demonstrated BKV DNA in 3.8% of 211 community children with a higher prevalence of subtype I. In HIV-1 positive children we detected BKV DNA in 2 urine samples (6%) out of 33, both belonging to subtype I. The HIV-negative cluster did not show any positivity to BKV DNA. The results confirm a more frequent primary BKV infection in children of 3-5 years of age and a higher prevalence in hospitalized children affected by HIV-1. The most relevant finding was that among both the community and HIV-1 positive children the subtype I was the most frequently detected.