Shadow effects in spiral phase contrast microscopy -: art. no. 233902
Shadow effects in spiral phase contrast microscopy -: art. no. 233902
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DOI：
10.1103/physrevlett.94.233902
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发表时间：
2005-06-17
影响因子：
8.6
通讯作者：
Ritsch-Marte, M
中科院分区：
文献类型：
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作者：
Jesacher, A;Fürhapter, S;Ritsch-Marte, M
最近已经证明，在光学路径的傅里叶平面螺旋相位元件的显微镜图像的空间滤波导致物体结构的强边缘增强。原则上，该操作是各向同性的，即采样对象的所有相位边缘同时突出显示，与它们的局部方向无关。然而，在这里，我们证明了通过控制螺旋相位全息图中心区域的相位可以有意地打破对称性，这是在计算机控制的空间光调制器上显示的。这产生了一个明显的阴影效果，可以以视频速率旋转。由此产生的浮雕样印象的样品地形与纵向分辨率在亚波长制度是通过成像一个标准的低对比度测试样品组成的人脸颊细胞。
Recently it has been demonstrated that spatial filtering of images in microscopy with a spiral phase element in a Fourier plane of the optical path results in a strong edge enhancement of object structures. In principle the operation is isotropic, i.e., all phase edges of a sample object are highlighted simultaneously, independent of their local direction. However, here we demonstrate that the symmetry can be broken intentionally by controlling the phase of the central area of a spiral phase hologram, which is displayed at a computer controlled spatial light modulator. This produces an apparent shadow effect which can be rotated at video rate. The resulting relieflike impression of the sample topography with a longitudinal resolution in the subwavelength regime is demonstrated by imaging a standard low contrast test sample consisting of a human cheek cell.