Cyanidin-3-Ο-glucoside supplementation in cryopreservation medium improves human sperm quality.
Cyanidin-3-Ο-glucoside supplementation in cryopreservation medium improves human sperm quality.
复制标题
在冷冻保存介质中添加花青素-3-β-葡萄糖苷可提高人类精子质量。
作者：
Xiaohan Wang;Xusheng Li;Yixun Liu;Xiaohua Jiang;Limin Wu;Ran Liu;Rentao Jin;Naru Zhou;Cheng Cao;Xuechun Hu;Bo Xu;Xianhong Tong;Weibin Bai;Shun Bai
目前，冷冻保存人类精子必须克服过度氧化的不利影响。在本研究中，我们旨在评估在冷冻保存培养基中添加花青素-3-Ο-glucoside （C3G）对精子质量的影响。根据WHO标准（n = 39）从正常精子症患者采集精液样本。比较在添加和不添加C3G的培养基中冷冻保存后精子的各项参数值。与对照组（无添加剂）相比，低剂量（50 μM和100 μM） C3G提高了精子活力和活力，降低了精子的活性氧（ROS），而高剂量（200 μM） C3G对精子质量的提高不明显。对照组冷冻后的DNA断裂指数（DFI）和高DNA染色率（HDS）均高于C3G添加组。低浓度C3G （50 μM）与精子ROS水平呈负相关（r = -0.2, p = 0.03）。总之，我们的研究结果表明，C3G可能是一种有效的精液冷冻保护剂，可以改善人类精子在冷冻保存过程中的氧化应激。
Currently, the cryopreservation of human spermatozoa must overcome the adverse effects of excessive oxidation. In this study, we aimed to evaluate the effect of supplementation of cryopreservation medium with cyanidin-3-Ο-glucoside (C3G) on sperm quality. Semen samples were obtained from men with normozoospermia according to WHO criteria (n = 39). The sperm parameter values were compared after cryopreservation in medium supplemented with and without C3G.Compared with the control group (without additive), low doses (50 μM and 100 μM) of C3G improved sperm viability and motility and decreased the reactive oxygen species (ROS) of spermatozoa, while high doses (200 μM) of C3G did not obviously enhance sperm quality. The amount of DNA fragmentation index (DFI) and high DNA stainability (HDS) after freezing were higher in the control group than in the C3G supplementation groups. Low-concentration C3G supplementation (50 μM) was negatively correlated with sperm ROS levels (r = -0.2, p = 0.03). Collectively, our findings suggest that C3G could be an efficient semen cryoprotectant that ameliorates oxidative stress in human sperm during cryopreservation.