Conformation of sturgeon pituitary somatotropin.
Conformation of sturgeon pituitary somatotropin.
复制标题
鲟鱼垂体生长激素的构象。
DOI：
10.1111/j.1399-3011.1987.tb02286.x
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Papkoff,H
中科院分区：
文献类型：
--
作者：
Bewley,TA;Papkoff,H
用零阶和二阶吸收光谱以及圆二色性研究了鲟鱼（Acipenser guldenstadti）的垂体生长激素（生长激素）。在激素的蛋白水解消化过程中也产生了不同的吸收光谱。在278.5 nm处，天然蛋白的摩尔消光系数为15 000±110 M‐1cm‐1。对鲟鱼生长激素和从牛、人等几种哺乳动物中分离的生长激素的构象进行了比较，结果表明这两种分子之间存在密切的关系。这种相似性可能与这种鱼类激素在哺乳动物试验系统中相对较高的生物效力有关。
Pituitary somatotropin (growth hormone) from the sturgeon (Acipenser guldenstadti) has been studied by zero‐order and second‐order absorption spectroscopy, as well as by circular dichroism. Difference absorption spectra have also been generated during proteolytic digestion of the hormone. The molar extinction coefficient of the native protein was found to be 15 000 ± 110 M‐1cm‐1at 278.5 nm. Comparison of the conformations of sturgeon somatotropin and somatotropins isolated from several mammalian species, including bovine and human, indicates a close relationship between these molecules. Such similarities may be related to the relatively high biopotency of this fish hormone in mammalian assay systems.