Protective effects of polydatin on experimental testicular torsion and detorsion injury in rats
Protective effects of polydatin on experimental testicular torsion and detorsion injury in rats
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虎杖甙对大鼠实验性睾丸扭转损伤的保护作用
DOI：
10.1071/rd17046
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Li Zhen
中科院分区：
文献类型：
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作者：
Qiao Huilian;Ma He;Cao Wanjun;Chen Hao;Wei Jinhua;Li Zhen
氧化应激在睾丸扭转复位（T/D）过程中起重要作用。本研究旨在探讨虎杖苷（PD）对睾丸T/D损伤的保护作用。将大鼠随机分为3组，假手术组、扭转2 h后复位24 h组和T/D组，在复位前30 min腹腔注射20 mg kg−1 PD。再灌注24 h后行单侧睾丸切除术。一半的睾丸进行组织学检查，苏木精-伊红染色和末端脱氧核糖核苷酸转移酶介导的dUTP-地高辛缺口末端标记（TUNEL）技术。在其余的组织中，丙二醛（MDA），过氧化氢酶（CAT），谷胱甘肽过氧化物酶（GPx）和超氧化物歧化酶（SOD）的水平进行了测定，因为是几个糖尿病相关蛋白的表达。与T/D组相比，PD预处理可明显改善睾丸形态学损伤，降低Cosentino组织学评分，增加睾丸生精细胞层数和Johnsen睾丸活检评分。此外，PD处理显著降低MDA水平，上调CAT、GPx和SOD活性。此外，PD还能降低T/D诱导的生殖细胞特异性凋亡，降低caspase-3、caspase-8、caspase-9和聚ADP核糖聚合酶的活性，增加Bcl-2/Bax比值。结果表明，PD对睾丸T/D损伤具有保护作用，尤其是在组织学、抗氧化应激和抗凋亡水平。
Oxidative stress plays a critical role in the process of testicular torsion and detorsion (T/D). The purpose of the present study was to investigate the protective effect of polydatin (PD) on testicular T/D injury. Rats were randomly divided into three groups, a sham group, a group subjected to 2 h torsion followed by 24 h detorsion and a group subjected to T/D and injected i.p. with 20 mg kg−1 PD 30 min before detorsion. Unilateral orchiectomy was performed after 24 h of reperfusion. Half the testes were prepared for histological examination by haematoxylin–eosin staining and the terminal deoxyribonucleotidyl transferase-mediated dUTP–digoxigenin nick end-labelling (TUNEL) technique. In the remaining tissues, levels of malondialdehyde (MDA), catalase (CAT), glutathione peroxidase (GPx) and superoxide dismutase (SOD) were determined, as was the expression of several apoptosis-related proteins. Compared with the T/D group, PD pretreatment significantly ameliorated the morphological damage, lowered the Cosentino histological score and increased the mean number of germ cell layers and Johnsen’s testicular biopsy score. In addition, PD treatment markedly decreased MDA levels and upregulated CAT, GPx and SOD activity. Furthermore, PD decreased T/D-induced germ cell-specific apoptosis, attenuated the activation of caspase-3, caspase-8, caspase-9 and poly(ADP-ribose) polymerase and increased the Bcl-2/Bax ratio. The findings indicate that PD has a protective effect against testicular T/D injuries, especially at the histological, antioxidative stress and antiapoptotic levels.