Laser scanning third-harmonic-generation microscopy in biology
Laser scanning third-harmonic-generation microscopy in biology
复制标题
DOI：
10.1364/oe.5.000169
复制
发表时间：
1999-10-11
期刊：
影响因子：
3.8
通讯作者：
Silberberg, Y
中科院分区：
文献类型：
--
作者：
Yelin, D;Silberberg, Y
使用三次谐波产生作为探针的激光扫描显微镜被证明可以产生透明生物标本的高分辨率图像。三次谐波光由紧密聚焦的短脉冲激光束产生，并逐点收集以形成数字图像。展示了两个生物样品。细胞培养物中的活神经元以清晰和详细的图像成像，包括光学分辨率阈值的细胞器。酵母细胞的内部细胞器也被成像，证明了该技术用于细胞和细胞内成像的能力。(C)1999年美国光学学会。
A laser scanning microscope using third-harmonic generation as a probe is shown to produce high-resolution images of transparent biological specimens. Third harmonic light is generated by a tightly focused short-pulse laser beam and collected point-by-point to form a digital image. Demonstrations with two biological samples are presented. Live neurons in a cell culture are imaged with clear and detailed images, including organelles at the threshold of optical resolution. Internal organelles of yeast cells are also imaged, demonstrating the ability of the technique for cellular and intracellular imaging. (C) 1999 Optical Society of America.