High glycogen levels in the hippocampus of patients with epilepsy
High glycogen levels in the hippocampus of patients with epilepsy
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DOI：
10.1038/sj.jcbfm.9600426
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发表时间：
2007-06-01
影响因子：
6.3
通讯作者：
Quistorff, Bjorn
中科院分区：
文献类型：
--
作者：
Dalsgaard, Mads K.;Madsen, Flemming F.;Quistorff, Bjorn
在强烈的脑激活过程中，人脑摄取的葡萄糖和乳酸盐中约有一半没有被氧化，可以补充糖原沉积，但人脑糖原浓度尚不清楚。在颞叶癫痫患者中，接受治疗性手术，从病理性海马（n = 19）和明显“正常”的皮质灰质和白色物质中获得脑活检。我们测定了在体脑糖原水平和糖原磷酸化酶和合成酶的活性。在健康猪（n = 5）中同样检查了糖原浓度的区域差异。在患者中，“正常”灰质和白色物质中的糖原浓度为5至6 mmol/ L，但海马体中的糖原浓度要高得多，为13.1 +/- 4.3 mmol/ L（平均值+/- s.d.;糖原磷酸化酶和糖原合成酶的活性变化趋势与此一致。在猪的正常海马体中，糖原同样高于灰质和白色物质。因此，在人类灰质和白色物质中，特别是在颞叶癫痫患者的海马体中，糖原构成了一个大的、活跃的能量储备，这对于癫痫发作时持续的突触活动期间的能量供应可能是重要的。
During intense cerebral activation approximately half of the glucose plus lactate taken up by the human brain is not oxidized and could replenish glycogen deposits, but the human brain glycogen concentration is unknown. In patients with temporal lobe epilepsy, undergoing curative surgery, brain biopsies were obtained from pathologic hippocampus (n = 19) and from apparently 'normal' cortical grey and white matter. We determined the in vivo brain glycogen level and the activity of glycogen phosphorylase and synthase. Regional differences in glycogen concentration were examined similarly in healthy pigs (n = 5). In the patients, the glycogen concentration in 'normal' grey and white matter was 5 to 6 mmol/ L, but much higher in the hippocampus, 13.1 +/- 4.3 mmol/ L (mean +/- s.d.; P < 0.001); the activities of glycogen phosphorylase and synthase displayed the same pattern. In normal hippocampus from pigs, glycogen was similarly higher than in grey and white matter. Consequently, in human grey and white matter and, particularly, in the hippocampus of patients with temporal lope epilepsy, glycogen constitutes a large, active energy reserve, which may be of importance for energy provision during sustained synaptic activity as epileptic seizures.