CD4+ T-cell response against human papillomavirus type 16 E6 protein is associated with a favorable clinical trend.
CD4+ T-cell response against human papillomavirus type 16 E6 protein is associated with a favorable clinical trend.
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DOI：
10.1007/s00262-011-1092-5
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发表时间：
2012-01
影响因子：
5.8
通讯作者：
Nakagawa, Mayumi
中科院分区：
文献类型：
--
作者：
Kim, Kevin H.;Greenfield, William W.;Cannon, Martin J.;Coleman, Hannah N.;Spencer, Horace J.;Nakagawa, Mayumi
CD8+ t细胞对人乳头瘤病毒16型（HPV-16） E6蛋白的反应与良好的临床趋势之间的关联已被证实。研究了人乳头瘤病毒（HPV）特异性CD4+ t细胞反应和调节性t细胞（Tregs）的作用。近期有异常帕氏涂片史的受试者符合条件，在入组时进行阴道镜引导活检。采用干扰素-γ酶联免疫斑点法和荧光活化细胞分选法测定Tregs频率。组织学诊断为宫颈上皮内瘤变1、2或3的受试者被认为有短期持续的宫颈异常，称为“持续者”（n=51），而组织学正常的受试者被称为“回归者”（n=33）。E6多肽的CD4+ t细胞应答率（15/33或45.5%）明显高于持续多肽（10/51或19.6%）（p= 0.015），而E7多肽则没有。某些E6区域的CD4+反应[E6(16-40), E6(91-115), E6（106-130）和E6(136-158)]在回归指标中也显着更高。虽然两组之间treg的频率没有差异，但treg的低频率与E6某些区域内CD4+ t细胞阳性反应显著相关[E6(16-40), E6(31-55), E6(76-100), E6(91-115), E6(106-130)]。CD4+和CD8+ t细胞对hpv - 16e6蛋白的应答与良好的临床趋势相关。HPV- 16e6蛋白应纳入HPV治疗性疫苗的设计中。
The association between the CD8+ T-cell responses to human papillomavirus type 16 (HPV-16) E6 protein and a favorable clinical trend has been demonstrated previously. The roles of human papillomavirus (HPV)-specific CD4+ T-cell responses and of regulatory T-cells (Tregs) were examined. Subjects with a recent history of abnormal Papanicolaou smear were eligible, and colposcopy guided biopsy was performed at enrollment. Interferon-γ enzyme-linked immunospot assay, and fluorescent activated cell sorter analysis to measure the frequencies of Tregs were performed. Subjects with histological diagnoses of cervical intraepithelial neoplasia 1, 2, or 3 were considered to have short-term persistence of cervical abnormality and were called “persistors” (n=51) while those of normal histology were designated to be “regressors” (n=33). A significantly higher percentage CD4+ T-cell response was detected in the regressors (15/33 or 45.5%) compared to the persistors (10/51 or 19.6%) (p=.015) for the E6 peptides but not for the E7 peptides. The CD4+ responses to certain E6 regions [E6(16–40), E6(91–115), E6(106–130), and E6(136–158)] were also significantly higher in the regressors. Although there was no difference in the frequencies of Tregs between the two groups, low frequencies of Tregs were significantly associated with positive CD4+ T-cell responses within certain E6 regions [E6(16–40), E6(31–55), E6(76–100), E6(91–115), and E6(106–130)]. The CD4+ and CD8+ T-cell responses to the HPV-16 E6 protein are associated with a favorable clinical trend. The HPV-16 E6 protein should be incorporated in the design of an HPV therapeutic vaccine.
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DOI：
10.2471/blt.06.038414
发表时间：
2007-09-01
期刊：
Bulletin of the World Health Organization: International Journal of Public Health
影响因子：
--
作者：
Cutts, FT;Franceschi, S;Markowitz, L
通讯作者：
Markowitz, L
影响因子：
6.4
作者：
Heusinkveld, Moniek;Welters, Marij J. P.;van der Burg, Sjoerd H.
通讯作者：
van der Burg, Sjoerd H.
影响因子：
5.5
作者：
Moscicki, Anna-Barbara;Schiffman, Mark;Villa, Luisa L.
通讯作者：
Villa, Luisa L.
影响因子：
158.5
作者：
KOUTSKY, LA;HOLMES, KK;KIVIAT, NB
通讯作者：
KIVIAT, NB
DOI：
10.1128/cdli.12.8.1003-1005.2005
发表时间：
2005-08-01
期刊：
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
影响因子：
--
作者：
Nakagawa, M;Kim, KH;Moscicki, AB
通讯作者：
Moscicki, AB