EXPRESSION OF APOLIPOPROTEIN-E DURING NERVE DEGENERATION AND REGENERATION
EXPRESSION OF APOLIPOPROTEIN-E DURING NERVE DEGENERATION AND REGENERATION
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DOI：
10.1073/pnas.83.4.1125
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发表时间：
1986-02-01
影响因子：
11.1
通讯作者：
SHOOTER, EM
中科院分区：
文献类型：
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作者：
IGNATIUS, MJ;GEBICKEHARTER, PJ;SHOOTER, EM
最近已经描述了一种37 kDA糖蛋白，其合成在大鼠坐骨神经和视神经损伤后大大增加。神经鞘中的细胞，损伤部位远端，产生并分泌大量蛋白质，因此，在损伤后3周，它代表了再生坐骨神经鞘的总可溶性细胞外蛋白的2-5％，尽管尽管它无法在受损的视神经中积聚。此处介绍的结果揭示了37 kDA神经损伤诱导的蛋白质与研究良好的血清蛋白载脂蛋白E（APOE）之间的同源性，该蛋白E（APOE）涉及脂质和胆固醇代谢，并且最近显示出在成人和成人和成人中存在。发展大鼠星形胶质细胞。两种蛋白质具有相同的等电聚焦点和相似的分子质量。针对37 kDa蛋白提出的抗体识别APOE和抗磷酸血清与37 kDa蛋白的抗体。 37 kDa蛋白匹配APOE的两个14个氨基酸拉伸的序列数据。这些数据表明37 kDa蛋白与血清APOE相同，并且可能具有与后者相似的功能。例如，在神经系统中，在发育期间和受伤后，脂质的修复，生长和维持中的脂质可能涉及脂质的动员和再利用。
A 37-kDa glycoprotein has been described recently, whose synthesis is dramatically increased after injury of the rat sciatic and optic nerves. Cells in the nerve sheath, distal to the site of injury, produce and secrete large amounts of this protein, so that by 3 weeks after injury, it represents 2-5% of the total soluble extracellular protein in the regenerating sciatic nerve sheath, although it fails to accumulate in damaged optic nerve. Results presented here reveal extensive homology between the 37-kDa nerve injury-induced protein and a well-studied serum protein, apolipoprotein E (apoE), that is involved in lipid and cholesterol metabolism and that has been shown recently to be present in adult and developing rat astroglia. Both proteins have identical isoelectric focusing points and similar molecular masses. Antibodies raised against the 37-kDa protein recognize apoE and anti-apoE serum crossreacts with the 37-kDa protein. Sequence data for two 14 amino acid stretches of the 37-kDa protein match identical regions of apoE. These data suggest that the 37-kDa protein is identical to serum apoE and that it could have similar functions to the latter. In the nervous system, for example, it may be involved in the mobilization and reutilization of lipid in the repair, growth, and maintenance of myelin and axonal membranes, both during development and after injury.