A Tunable Silk Hydrogel Device for Studying Limb Regeneration in Adult Xenopus Laevis
A Tunable Silk Hydrogel Device for Studying Limb Regeneration in Adult Xenopus Laevis
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DOI：
10.1371/journal.pone.0155618
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发表时间：
2016-06-03
期刊：
影响因子：
3.7
通讯作者：
Kaplan, David L.
中科院分区：
文献类型：
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作者：
Golding, Anne;Guay, Justin A.;Kaplan, David L.
在某些两栖动物模型中，肢体再生可以由局部伤口床环境促进或抑制。本研究介绍了一种装置，可用作实验工具，以表征条件，促进肢体再生的成年青蛙（非洲爪蟾）模型。特别是，该器械设计用于通过水凝胶插入物控制局部伤口环境。水凝胶插入物的初始表征显示，由于水凝胶的收缩，这种相互作用对动物的机械力具有显著影响。水凝胶衬垫的材料和机械性能是器械设计中与动物舒适度和有效操作截肢部位的能力相关的一个因素。水凝胶的可调特征在确定肢体再生中的促再生作用中是重要的，这通过软骨棘突形成来测量，并通过显微计算机断层扫描来量化。水凝胶插入物是截肢后观察到的形态结果的一个因素。未来的工作将集中在表征和优化该设备的观察能力，以操纵肢体再生所必需的生物途径。然而，目前的工作为水凝胶在设备中的作用提供了一个框架，并为更系统的研究提供了一条前进的道路。
In certain amphibian models limb regeneration can be promoted or inhibited by the local wound bed environment. This research introduces a device that can be utilized as an experimental tool to characterize the conditions that promotes limb regeneration in the adult frog ( Xenopus laevis) model. In particular, this device was designed to manipulate the local wound environment via a hydrogel insert. Initial characterization of the hydrogel insert revealed that this interaction had a significant influence on mechanical forces to the animal, due to the contraction of the hydrogel. The material and mechanical properties of the hydrogel insert were a factor in the device design in relation to the comfort of the animal and the ability to effectively manipulate the amputation site. The tunable features of the hydrogel were important in determining the pro-regenerative effects in limb regeneration, which was measured by cartilage spike formation and quantified by micro-computed tomography. The hydrogel insert was a factor in the observed morphological outcomes following amputation. Future work will focus on characterizing and optimizing the device's observed capability to manipulate biological pathways that are essential for limb regeneration. However, the present work provides a framework for the role of a hydrogel in the device and a path forward for more systematic studies.