Adipogenic cascade can be induced without adipogenic media by a human adenovirus.
Adipogenic cascade can be induced without adipogenic media by a human adenovirus.
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DOI：
10.1038/oby.2008.630
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发表时间：
2009-04
期刊：
影响因子：
6.9
通讯作者：
Dhurandhar, Nikhil V.
中科院分区：
文献类型：
--
作者：
Rathod, Miloni A.;Rogers, Pamela M.;Vangipuram, Sharada D.;McAllister, Emily J.;Dhurandhar, Nikhil V.
一些代谢异常与脂肪组织的相对过剩或不足有关。识别成脂分化的调控因子是其成功操作的关键。AD36是一种人腺病毒，是一种促进脂肪生成的新因子。我们利用AD36的成脂潜力来揭示在有或没有分化诱导剂甲基-异丁基-黄嘌呤、地塞米松和胰岛素(M、D和I)的情况下，外源成脂调节剂对啮齿动物前脂肪细胞系的成脂作用。用非成脂人腺病毒AD2作为病毒感染的阴性对照。首先，我们证实，AD36，而不是AD2，在存在或不存在MDI的情况下会增加脂质积累。成脂通路关键基因表达的时程研究表明，Ad36下调前脂肪细胞标志基因WNT10b的表达，而上调早期(C/eBPδ和C/eBPβ)、中期(PPARγ2)和晚期(aP2和G3PDH)基因的表达。在MDI存在的情况下，AD36组和对照组成脂基因的表达开始一致，但AD36组的表达明显更多。接下来，我们观察到抗腺病毒药物对AD36 mRNA表达的抑制降低了3T3-L1分化，表明这一过程需要病毒mRNA的表达。此外，无论有没有MDI或其成分，AD36都显著增加了3T3-L1细胞中的脂质积累。AD36感染时细胞融合对脂质积聚有正向影响。结果表明，AD36是一种MDI非依赖性的外源性成脂调节因子。阐明相关的分子途径可能揭示脂肪形成的新的调控机制。
Several metabolic abnormalities are associated with relative excess or deficiency of adipose tissue. Identifying the regulators of adipogenic differentiation is critical for its successful manipulation. Ad36, a human adenovirus, is a novel factor that promotes adipogenesis. We exploited the adipogenic potential of Ad36 to reveal exogenous modifiers of adipogenesis in rodent preadipocyte cell line in presence or absence of differentiation inducers methyl-isobutyl-xanthine, dexamethasone, and insulin (M, D, and I). A non-adipogenic human adenovirus Ad2 was used as a negative control for viral infection. First, we confirmed that, Ad36, but not Ad2, increases lipid accumulation in presence or absence of MDI. Time course studies for expression of key genes of adipogenic cascade showed that Ad36, but not Ad2 down regulated preadipocyte marker gene Wnt10b, and up regulated expression of early (C/EBPδ and C/EBPβ), intermediate (PPARγ2) and late genes (aP2 and G3PDH) of adipogenic cascade even in the absence of MDI. In presence of MDI, onset of expression of adipogenic genes coincided for Ad36 and control groups, but the expressions were significantly greater for the Ad36 group. Next, we observed that attenuation of Ad36 mRNA expression by an anti-adenoviral agent reduced 3T3-L1 differentiation, indicating that viral mRNA expression is required for the process. Furthermore, with or without MDI or its components, Ad36 significantly increased lipid accumulation in 3T3-L1 cells. Cell confluency at the time of Ad36 infection positively influenced lipid accumulation. The results reveal that Ad36 is an MDI-independent exogenous regulator of the adipogenic process. Elucidating the molecular pathways involved may reveal novel regulatory controls of adipogenesis.
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DOI：
10.1073/pnas.93.11.5507
发表时间：
1996-05-28
影响因子：
11.1
作者：
Miller, SG;DEVos, P;Auwerx, J
通讯作者：
Auwerx, J
DOI：
10.1016/s1043-4526(06)52002-9
发表时间：
2007-01-01
期刊：
ADVANCES IN FOOD AND NUTRITION RESEARCH, VOL 52
影响因子：
--
作者：
Pasarica, Magdalena;Dhurandhar, Nikhil V.
通讯作者：
Dhurandhar, Nikhil V.
影响因子：
4.9
作者：
Farmer, SR
通讯作者：
Farmer, SR
影响因子：
4.9
作者：
Rogers, P. M.;Fusinski, K. A.;Dhurandhar, N. V.
通讯作者：
Dhurandhar, N. V.
影响因子：
4.9
作者：
Vangipuram, S. D.;Yu, M.;Dhurandhar, N. V.
通讯作者：
Dhurandhar, N. V.