Maladaptive Contractility of 3D Human Cardiac Microtissues to Mechanical Nonuniformity
Maladaptive Contractility of 3D Human Cardiac Microtissues to Mechanical Nonuniformity
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DOI：
10.1002/adhm.201901373
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发表时间：
2020-02
影响因子：
10
通讯作者：
Chenyan Wang;Sangmo Koo;Minok Park;Z. Vangelatos;Plansky Hoang;B. Conklin;C. Grigoropoulos;K. Healy;Zhen Ma
中科院分区：
文献类型：
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作者：
Chenyan Wang;Sangmo Koo;Minok Park;Z. Vangelatos;Plansky Hoang;B. Conklin;C. Grigoropoulos;K. Healy;Zhen Ma
心脏组织能够调整其收缩行为以适应局部机械环境。天然组织力学性质的不均匀性有助于心脏功能障碍的发展，但目前的体外心脏组织模型往往无法重现力学不均匀性。为了解决这个问题，开发了一种具有工程机械不均匀性的3D心脏微组织模型，该模型由由不同直径的纤维组成的3D打印混合基质实现。当组织力学环境的复杂性升级时，心脏微组织开始发展适应不良的过度收缩表型，在收缩运动分析和力-功率分析中均得到证实。这种新型的混合系统可能有助于建立“病理启发”的心脏微组织模型，以更深入地了解由于组织力学环境的不均匀性而导致的心脏病理。
Cardiac tissues are able to adjust their contractile behavior to adapt to the local mechanical environment. Nonuniformity of the native tissue mechanical properties contributes to the development of heart dysfunctions, yet the current in vitro cardiac tissue models often fail to recapitulate the mechanical nonuniformity. To address this issue, a 3D cardiac microtissue model is developed with engineered mechanical nonuniformity, enabled by 3D‐printed hybrid matrices composed of fibers with different diameters. When escalating the complexity of tissue mechanical environments, cardiac microtissues start to develop maladaptive hypercontractile phenotypes, demonstrated in both contractile motion analysis and force‐power analysis. This novel hybrid system could potentially facilitate the establishment of “pathologically‐inspired” cardiac microtissue models for deeper understanding of heart pathology due to nonuniformity of the tissue mechanical environment.