THTA, a thermotolerance gene of Aspergillus fumigatus
THTA, a thermotolerance gene of Aspergillus fumigatus
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DOI：
10.1016/j.fgb.2004.06.004
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发表时间：
2004-09-01
影响因子：
3
通讯作者：
Kwon-Chung, KJ
中科院分区：
文献类型：
--
作者：
Chang, YC;Tsai, HF;Kwon-Chung, KJ
烟曲霉最适生长温度为37至42摄氏度，但可在55摄氏度以下生长。为了研究烟曲霉耐热性的遗传基础及其在致病力中的作用，筛选了温度敏感突变体。其中一个在42摄氏度而不是48摄氏度生长的突变体被互补，基因Tha。已被确认。缺失的thta表现出与原始突变体相同的温度敏感性。THTA编码一种功能未知的141 kDa蛋白质，HA标记的ThtAp在37℃或48℃培养中积累到类似水平。Southern杂交分析和数据库搜索显示，在其他几种子囊菌中存在与thta相关的序列。DeltathtA菌株和野生型菌株之间的毒力没有差异。因此，这对烟曲霉菌在高温下的生长是必不可少的，但对该物种的致病性没有贡献。由爱思唯尔公司出版。
Aspergillus fumigatus grows optimally from 37 to 42degreesC but can grow at temperatures up to 55 degreesC. To study the genetic basis of thermotolerance and its role in virulence of A. fumigatus, temperature sensitive mutants were isolated. One of the mutants that grew at 42degreesC but not at 48degreesC was complemented and the gene, THTA. was identified. Deletion of THTA showed the same temperature sensitivity as the original mutant. THTA encodes a putative protein of 141 kDa with unknown function and the HA-tagged ThtAp accumulated to similar levels in cultures grown at either 37 or 48degreesC. Southern blot analysis and database searches revealed the presence of THTA-related sequences in several other ascomycetous fungi. No difference in virulence was observed between the DeltathtA and wild-type strains. Thus, THTA is essential for growth of A. fumigatus at high temperatures but does not contribute to the pathogenicity of the species. Published by Elsevier Inc.