Antagonistic roles of Wnt5 and the Drl receptor in patterning the Drosophila antennal lobe
Antagonistic roles of Wnt5 and the Drl receptor in patterning the Drosophila antennal lobe
复制标题
DOI：
10.1038/nn1993
复制
发表时间：
2007-11-01
影响因子：
25
通讯作者：
Hing，Huey
中科院分区：
文献类型：
--
作者：
Yao，Ying;Wu，Yuping;Hing，Huey
大量研究表明，向内生长的嗅觉轴突对嗅觉图的发育产生强大的诱导影响。通过过表达筛选，我们鉴定出 wnt5 是果蝇嗅觉图谱的有效组织者。 wnt5的缺失导致肾小球模式的严重紊乱，而wnt5的过度表达导致异位中线肾小球的形成。细胞类型特异性 cDNA 拯救和嵌合实验表明 wnt5 在嗅觉神经元中发挥作用。编码 Wnt5 受体的 derailed(drl) 基因突变，导致肾小球图谱紊乱、异位中线肾小球以及 Wnt5 在中线积聚。我们在这里展示了 drl 在神经胶质细胞中发挥作用，它在 wnt5 的上游发挥作用，调节其在肾小球模式中的功能。我们的研究结果确立了wnt5是一种由嗅觉轴突表达的顺行信号，并证明了神经胶质细胞在果蝇嗅觉图谱形成过程中的先前未被认识但强大的作用。
Numerous studies have shown that ingrowing olfactory axons exert powerful inductive influences on olfactory map development. From an overexpression screen, we have identifiedwnt5as a potent organizer of the olfactory map inDrosophila melanogaster. Loss ofwnt5resulted in severe derangement of the glomerular pattern, whereas overexpression ofwnt5resulted in the formation of ectopic midline glomeruli. Cell type–specific cDNA rescue and mosaic experiments showed thatwnt5functions in olfactory neurons. Mutation of thederailed(drl) gene, encoding a receptor for Wnt5, resulted in derangement of the glomerular map, ectopic midline glomeruli and the accumulation of Wnt5 at the midline. We show here thatdrlfunctions in glial cells, where it acts upstream ofwnt5to modulate its function in glomerular patterning. Our findings establishwnt5as an anterograde signal that is expressed by olfactory axons and demonstrate a previously unappreciated, yet powerful, role for glia in patterning theDrosophilaolfactory map.