Functional analysis of the A complementation group mutants of Sindbis HR virus.
Functional analysis of the A complementation group mutants of Sindbis HR virus.
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Sindbis HR病毒A互补组突变体的功能分析。
DOI：
10.1016/0042-6822(85)90301-0
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发表时间：
1985
期刊：
影响因子：
3.7
通讯作者：
Sawicki,SG
中科院分区：
文献类型：
--
作者：
Sawicki,DL;Sawicki,SG
辛德毕斯病毒耐热株SIN HR的温度敏感突变体A互补组的10个成员被分为两个表型亚组。亚组I突变体（ts 15、ts 17、ts 21、ts 24和ts 133）表现出温度敏感的26 S mRNA合成，而亚组II突变体（ts 4、ts 14、ts 16、ts 19和ts 138）则没有; ts 4和ts 19在30°下均表现出缺陷的26 S mRNA合成。没有一个A组突变体表现出温度敏感的49 S正链合成。温度敏感的切割ns 230被证明由亚组I突变体，除了ts 21，但不是由亚组II突变体。在40°生长的回复突变体ts 133保留了温度敏感的26 S mRNA合成，但失去了ns 230的温度敏感切割和RNA阴性表型。只有ts 4，像B互补组的tsll，表现出温度敏感的负链RNA合成。除了ts 24，感染ts 17或ts 133的细胞继续合成负链后，shiftweek在没有持续的蛋白质合成，并恢复负链的合成，如果转移到非允许的温度后，负链的合成已经停止在允许的温度。
The 10 members of the A complementation group of temperature-sensitive (ts) mutants of SIN HR, the heat-resistant strain of Sindbis virus, were divided into two phenotypic subgroups. Subgroup I mutants (ts15, ts17, ts21, ts24, and ts133) demonstrated temperature-sensitive 26 S mRNA synthesis, whereas subgroup II mutants (ts4, ts14, ts16, ts19, and ts138) did not; both ts4 and ts19 demonstrated defective 26 S mRNA synthesis at 30°. None of the A group mutants demonstrated temperature-sensitive 49 S plus-strand synthesis. Temperature-sensitive cleavage of ns230 was demonstrated by subgroup I mutants, except ts21, but not by subgroup II mutants. A revertant of ts133 that grew at 40° retained temperature-sensitive 26 S mRNA synthesis but lost temperature-sensitive cleavage of ns230 and the RNA-negative phenotype. Only ts4, like tsll of the B complementation group, demonstrated temperature-sensitive minus-strand RNA synthesis. In addition to ts24, cells infected with ts17 or ts133 continued to synthesize minus strands after shiftup in the absence of continued protein synthesis, and resumed synthesis of minus strands if shifted to the nonpermissive temperature after minus-strand synthesis had ceased at the permissive temperature.