Interaction of fibroblasts and polymer surfaces: relationship between surface free energy and fibroblast spreading.
Interaction of fibroblasts and polymer surfaces: relationship between surface free energy and fibroblast spreading.
复制标题
成纤维细胞和聚合物表面的相互作用：表面自由能和成纤维细胞扩散之间的关系。
DOI：
10.1002/jbm.820170508
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
J. Arends
中科院分区：
文献类型：
--
作者：
P. Van Der Valk;A. van Pelt;H. Busscher;H. de Jong;C. Wildevuur;J. Arends
在血清蛋白存在下，研究了小鼠肺成纤维细胞转化系在各种聚合物表面上的细胞铺展和细胞分裂速率。裸露的聚合物表面，以及蛋白质涂覆的表面，其特征在于其极性（γ ps）和分散（γ ds）的表面自由能。细胞铺展似乎是依赖于极性表面自由能。当裸露表面的γ ps低于5 erg cm-2时，细胞扩展较低;当γ ps高于15 erg cm-2时，发生显著的扩展。发现细胞扩散和蛋白质包被表面的极性之间存在类似的关系，尽管不如裸露表面明显。细胞扩散出现独立的分散表面自由能。细胞分裂速率在所有测试表面上是相同的。
Cell spreading and cell division rates of a transformed line of mouse lung fibroblasts were studied on various polymer surfaces in the presence of serum proteins. The bare polymer surfaces, as well as the protein-coated surfaces, were characterized by their polar (gamma ps) and dispersion (gamma ds) surface free energies. Cell spreading appeared to be dependent on the polar surface free energy. Cell spreading is low when the gamma ps of the bare surface is lower than 5 erg cm-2; marked spreading occurs when gamma ps is higher than 15 erg cm-2. A similar relationship was found between cell spreading and polarity of the protein-coated surfaces, although less pronounced than for the bare surfaces. Cell spreading appeared independent of the dispersion surface free energy. Cell division rate was the same on all surfaces tested.