Genetically Inherited Obesity and High-Fat Diet-Induced Obesity Differentially Alter Spermatogenesis in Adult Male Rats
Genetically Inherited Obesity and High-Fat Diet-Induced Obesity Differentially Alter Spermatogenesis in Adult Male Rats
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DOI：
10.1210/en.2018-00569
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发表时间：
2019-01-01
期刊：
影响因子：
4.8
通讯作者：
Balasinor, Nafisa H.
中科院分区：
文献类型：
--
作者：
Deshpande, Sharvari S.;Nemani, Harishankar;Balasinor, Nafisa H.
肥胖是一种多因素疾病，主要是遗传和/或环境原因。我们的目的是利用两种Wistar大鼠模型：遗传遗传性肥胖（GIO） WNIN/Ob大鼠和饮食性肥胖（DIO）大鼠，研究遗传遗传性和高脂肪饮食诱导的肥胖对生育和精子发生的影响。两组的终末体重相似，但代谢和激素谱在两组之间有显著差异。生育能力评估显示，由于植入前和植入后的损失增加，DIO组的产仔数显著减少，而GIO组的大鼠由于缺乏性欲而不育。与DIO组相比，GIO组的精子数量明显减少。基于倍性和细胞类型特异性表达标记对睾丸细胞进行计数，研究肥胖对精子发生的影响，发现GIO和DIO状态影响有丝分裂：精原细胞和s期群体增加。然而，两组对减数分裂和精子发生的影响不同。GIO和DIO对生育能力和精子发生的不同影响可能是由于两组之间白色脂肪组织积累的显著差异，而不是由于高体重。肥胖的不同影响表明，在人类中观察到的男性肥胖引起的不育症可能是遗传和环境因素的结合。
Obesity is a multifactorial disorder with predominantly genetic and/or environmental causes. Our aim was to delineate effects of genetically inherited and high-fat diet-induced obesity on fertility and spermatogenesis using two Wistar rat models: genetically inherited obese (GIO) WNIN/Ob rats and diet-induced obese (DIO) rats, which received a high-fat diet. The terminal body weights were similar in both groups, but there was a significant difference in metabolic and hormone profiles between the groups. Fertility assessment revealed a significant decrease in the litter size due to increased pre- and postimplantation loss in the DIO group, whereas the rats in the GIO group were infertile due to lack of libido. Significantly decreased sperm counts were observed in the GIO group compared with the DIO group. Enumeration of testicular cells on the basis of ploidy and cell type-specific expression markers, to study the effect of obesity on spermatogenesis, demonstrated that the GIO and DIO states affected mitosis: spermatogonia and S-phase population were increased. However, distinctive effects were observed on meiosis and spermiogenesis in both the groups. Differential effects of GIO and DIO on fertility and spermatogenesis could be due to the significant difference in white adipose tissue accumulation between the groups and not due to high body weights. The differential effects of obesity suggest male obesity-induced infertility observed in humans could be a combination of genetic and environmental factors.