Lipopolysaccharide O-chain core region required for cellular cohesion and compaction of in vitro and root biofilms developed by Rhizobium leguminosarum.
Lipopolysaccharide O-chain core region required for cellular cohesion and compaction of in vitro and root biofilms developed by Rhizobium leguminosarum.
复制标题
豆根瘤菌形成的体外和根生物膜的细胞凝聚和压实所需的脂多糖 O 链核心区域。
DOI：
10.1128/aem.03175-14
复制
发表时间：
2015
影响因子：
4.4
通讯作者：
Russo DM
中科院分区：
文献类型：
--
作者：
Russo DM
生物膜的形成是根瘤菌在土壤颗粒和植物根部生存的重要策略。在由根瘤菌形成的生物膜的小菌落内，根瘤菌细胞通过侧向和极性连接紧密地相互作用，形成有组织的和紧凑的细胞聚集体。这些小菌落嵌入生物膜基质中，其主要成分是酸性胞外多糖（EPS）。我们的工作表明，O链的核心区域的R。豆胶脂多糖（LPS）（从细胞表面伸出）强烈影响细菌粘附特性和细胞间凝聚力。O链或O链核心部分缺陷的突变体产生了过早的小菌落，其中细菌的侧向接触大大减少。此外，LPS突变体的小菌落内的细胞-细胞相互作用主要通过它们的极介导，导致生物膜具有改变的三维结构和增加的厚度。此外，在根表皮和根毛上，O-抗原核心缺陷株表现出改变的生物膜模式与典型的小菌落压实受损。总之，这些结果表明，LPS的表面暴露部分对于适当的细胞间相互作用和在不同表面上形成稳健的生物膜至关重要。
The formation of biofilms is an important survival strategy allowing rhizobia to live on soil particles and plant roots. Within the microcolonies of the biofilm developed by Rhizobium leguminosarum, rhizobial cells interact tightly through lateral and polar connections, forming organized and compact cell aggregates. These microcolonies are embedded in a biofilm matrix, whose main component is the acidic exopolysaccharide (EPS). Our work shows that the O-chain core region of the R. leguminosarum lipopolysaccharide (LPS) (which stretches out of the cell surface) strongly influences bacterial adhesive properties and cell-cell cohesion. Mutants defective in the O chain or O-chain core moiety developed premature microcolonies in which lateral bacterial contacts were greatly reduced. Furthermore, cell-cell interactions within the microcolonies of the LPS mutants were mediated mostly through their poles, resulting in a biofilm with an altered three-dimensional structure and increased thickness. In addition, on the root epidermis and on root hairs, O-antigen core-defective strains showed altered biofilm patterns with the typical microcolony compaction impaired. Taken together, these results indicate that the surface-exposed moiety of the LPS is crucial for proper cell-to-cell interactions and for the formation of robust biofilms on different surfaces.